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Bombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee crop

2015Journal article Restricted access
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dc.contributor.authorLi, Leilei
dc.contributor.authorPraet, Jessy
dc.contributor.authorBorremans, Wim
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.contributor.authorCleenwerck, Ilse
dc.contributor.authorMeeus, Ivan
dc.contributor.authorSmagghe, Guy
dc.contributor.authorDe Vuyst, Luc
dc.contributor.authorVandamme, Peter
dc.date.accessioned2016-04-21T14:34:26Z
dc.date.available2016-04-21T14:34:26Z
dc.date.issued2015
dc.description.abstractIn the frame of a bumble bee gut microbiota study, acetic acid bacteria (AAB) were isolated using a combination of direct isolation methods and enrichment procedures. MALDI-TOF MS profiling of the isolates and a comparison of these profiles with profiles of established AAB species identified most isolates as Asaia astilbis or as 'Commensalibacter intestini', except for two isolates (R-52486 and LMG 28161(T)) that showed an identical profile. A nearly complete 16S rRNA gene sequence of strain LMG 28161(T) was determined and showed the highest pairwise similarity to Saccharibacter floricola S-877(T) (96.5 %), which corresponded with genus level divergence in the family Acetobacteraceae. Isolate LMG 28161(T) was subjected to whole-genome shotgun sequencing; a 16S-23S rRNA internal transcribed spacer (ITS) sequence as well as partial sequences of the housekeeping genes dnaK, groEL and rpoB were extracted for phylogenetic analyses. The obtained data confirmed that this isolate is best classified into a new genus in the family Acetobacteraceae. The DNA G + C content of strain LMG 28161(T) was 54.9 mol%. The fatty acid compositions of isolates R-52486 and LMG 28161(T) were similar to those of established AAB species [with C-18:1 omega 7c (43.1%) as the major component], but the amounts of fatty acids such as C-19:0 cyclo omega 8c, C-14:0 and C-14:0 2-OH enabled to differentiate them. The major ubiquinone was Q-10. Both isolates could also be differentiated from the known genera of AAB by means of biochemical characteristics, such as their inability to oxidize ethanol to acetic acid, negligible acid production from melibiose, and notable acid production from D-fructose, sucrose and D-mannitol. In addition, they produced 2-keto-D-gluconate, but not 5-keto-D-gluconate from D-glucose. Therefore, the name Bombella intestini gen nov., sp. nov. is proposed for this new taxon, with LMG 28161(T) (=DSM 28636(T)=R-52487(T)) as the type strain of the type species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationLI, Leilei; PRAET, Jessy; BORREMANS, Wim; NUNES, Olga; MANAIA, Célia; CLEENWERCK, Ilse; MEEUS, Ivan; SMAGGHE, Guy; VUYST, Luc De; VANDAMME, Peter - Bombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee crop. International Journal of Systematic and Evolutionary Microbiology. ISSN 1466-5026. Vol. 65 (2015), p. 267–273pt_PT
dc.identifier.doi10.1099/ijs.0.068049-0
dc.identifier.eid84921512280
dc.identifier.eissn1466-5034
dc.identifier.issn1466-5026
dc.identifier.pmid25336723
dc.identifier.urihttp://hdl.handle.net/10400.14/19903
dc.identifier.wos000355360000042
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMicrobiology Society
dc.titleBombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee croppt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage273
oaire.citation.issue1
oaire.citation.startPage267
oaire.citation.titleInternational Journal of Systematic and Evolutionary Microbiology
oaire.citation.volume65
person.familyNameLi
person.familyNameNunes
person.familyNameManaia
person.familyNameSmagghe
person.familyNameVandamme
person.givenNameLeilei
person.givenNameOlga
person.givenNameCélia
person.givenNameGuy
person.givenNamePeter
person.identifier336509
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0003-0185-510X
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-8334-3313
person.identifier.orcid0000-0002-5581-7937
person.identifier.ridB-7967-2009
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
person.identifier.scopus-author-id56211244100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa59f766e-8906-46e6-aae4-65fa681170b1
relation.isAuthorOfPublication6b4a1abf-b245-41ad-8335-fa0f379650bb
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relation.isAuthorOfPublication.latestForDiscoverya59f766e-8906-46e6-aae4-65fa681170b1

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