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Cell-to-cell transmission of HIV-1 and HIV-2 from infected macrophages and dendritic cells to CD4+ T lymphocytes

2023-04-22Journal article Open access
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dc.contributor.authorCalado, Marta
dc.contributor.authorPires, David
dc.contributor.authorConceição, Carolina
dc.contributor.authorFerreira, Rita
dc.contributor.authorSantos-Costa, Quirina
dc.contributor.authorAnes, Elsa
dc.contributor.authorAzevedo-Pereira, José Miguel
dc.date.accessioned2023-06-14T08:48:20Z
dc.date.available2023-06-14T08:48:20Z
dc.date.issued2023-04-22
dc.description.abstractMacrophages (Mø) and dendritic cells (DCs) are key players in human immunodeficiency virus (HIV) infection and pathogenesis. They are essential for the spread of HIV to CD4+ T lymphocytes (TCD4+) during acute infection. In addition, they constitute a persistently infected reservoir in which viral production is maintained for long periods of time during chronic infection. Defining how HIV interacts with these cells remains a critical area of research to elucidate the pathogenic mechanisms of acute spread and sustained chronic infection and transmission. To address this issue, we analyzed a panel of phenotypically distinct HIV-1 and HIV-2 primary isolates for the efficiency with which they are transferred from infected DCs or Mø to TCD4+. Our results show that infected Mø and DCs spread the virus to TCD4+ via cell-free viral particles in addition to other alternative pathways. We demonstrate that the production of infectious viral particles is induced by the co-culture of different cell populations, indicating that the contribution of cell signaling driven by cell-to-cell contact is a trigger for viral replication. The results obtained do not correlate with the phenotypic characteristics of the HIV isolates, namely their co-receptor usage, nor do we find significant differences between HIV-1 and HIV-2 in terms of cis- or trans-infection. The data presented here may help to further elucidate the cell-to-cell spread of HIV and its importance in HIV pathogenesis. Ultimately, this knowledge is critical for new therapeutic and vaccine approaches.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/v15051030pt_PT
dc.identifier.eid85160377912
dc.identifier.issn1999-4915
dc.identifier.pmcPMC10222233
dc.identifier.pmid37243118
dc.identifier.urihttp://hdl.handle.net/10400.14/41354
dc.identifier.wos000998095600001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAcute infectionpt_PT
dc.subjectCis-infectionpt_PT
dc.subjectDendritic cellspt_PT
dc.subjectHIVpt_PT
dc.subjectMacrophagespt_PT
dc.subjectTrans-infectionpt_PT
dc.subjectTransmissionpt_PT
dc.titleCell-to-cell transmission of HIV-1 and HIV-2 from infected macrophages and dendritic cells to CD4+ T lymphocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleVirusespt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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