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Persistence of wastewater antibiotic resistant bacteria and their genes in human fecal material

2020Journal article Open access
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dc.contributor.authorScaccia, Nazareno
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2020-04-17T10:56:27Z
dc.date.available2020-04-17T10:56:27Z
dc.date.issued2020
dc.description.abstractDomestic wastewater is a recognized source of antibiotic resistant bacteria and antibiotic resistance genes (ARB&ARGs), whose risk of transmission to humans cannot be ignored. The fitness of wastewater ARB in the complex fecal microbiota of a healthy human was investigated in feces-based microcosm assays (FMAs). FMAs were inoculated with two wastewater isolates, Escherichia coli strain A2FCC14 (MLST ST131) and Enterococcus faecium strain H1EV10 (MLST ST78), harboring the ARGs blaTEM, blaCTX, blaOXA-A and vanA, respectively. The FMAs, incubated in the presence or absence of oxygen or in the presence or absence of the antibiotics cefotaxime or vancomycin, were monitored based on cultivation, ARGs quantification and bacterial community analysis. The fecal bacterial community was dominated by members of the phyla Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The ARGs harbored by the wastewater isolates could be quantified after one week, in FMAs incubated under both aerobic and anaerobic conditions. These observations were not significantly different in FMAs incubated anaerobically, supplemented with sub-inhibitory concentrations of cefotaxime or vancomycin. The observation that ARGs of wastewater ARB persisted in presence of the human fecal microbiota for at least one week supports the hypothesis of a potential transmission to humans, a topic that deserves further investigation.pt_PT
dc.description.versioninfo:eu-repo/semantics/submittedVersionpt_PT
dc.identifier.citationScaccia, N., Vaz-Moreira, I., Manaia, C. M. (2020). Persistence of wastewater antibiotic resistant bacteria and their genes in human fecal material. FEMS Microbiology Ecologypt_PT
dc.identifier.doi10.1093/femsec/fiaa058pt_PT
dc.identifier.eid85085715235
dc.identifier.eissn1574-6941
dc.identifier.issn0168-6496
dc.identifier.pmid32239211
dc.identifier.urihttp://hdl.handle.net/10400.14/30277
dc.identifier.wos000565902700003
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation675530pt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHuman fecal microbiotapt_PT
dc.subjectMicrocosm assayspt_PT
dc.subjectMicrocosm effectpt_PT
dc.subjectAntibiotic resistance transmissionpt_PT
dc.subjectAntibiotic resistant bacteriapt_PT
dc.subjectAntibiotic resistance genespt_PT
dc.titlePersistence of wastewater antibiotic resistant bacteria and their genes in human fecal materialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.titleFEMS Microbiology Ecologypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameScaccia
person.familyNameVaz-Moreira
person.familyNameManaia
person.givenNameNazareno
person.givenNameIvone
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0003-3294-3815
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication558ab291-9135-47ce-8f2c-6f052381b5b5
relation.isAuthorOfPublicationf3e78f4a-1586-4e5c-a182-81009f20915a
relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
relation.isAuthorOfPublication.latestForDiscoveryf3e78f4a-1586-4e5c-a182-81009f20915a
relation.isProjectOfPublicationd552b32e-c55f-428f-a51c-1401fdf33697
relation.isProjectOfPublication.latestForDiscoveryd552b32e-c55f-428f-a51c-1401fdf33697

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