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Genomic and transcriptomic analysis of Saccharomyces cerevisiae isolates with focus in succinic acid production

2017Journal article Restricted access
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dc.contributor.authorFranco-Duarte, Ricardo
dc.contributor.authorBessa, Daniela
dc.contributor.authorGonçalves, Filipa
dc.contributor.authorMartins, Rosa
dc.contributor.authorSilva-Ferreira, António César
dc.contributor.authorSchuller, Dorit
dc.contributor.authorSampaio, Paula
dc.contributor.authorPais, Célia
dc.date.accessioned2018-11-06T18:19:10Z
dc.date.available2018-11-06T18:19:10Z
dc.date.issued2017
dc.description.abstractSuccinic acid is a platform chemical that plays an important role as precursor for the synthesis of many valuable bio-based chemicals. Its microbial production from renewable resources has seen great developments, specially exploring the use of yeasts to overcome the limitations of using bacteria. The objective of the present work was to screen for succinate-producing isolates, using a yeast collection with different origins and characteristics. Four strains were chosen, two as promising succinic acid producers, in comparison with two low producers. Genome of these isolates was analysed, and differences were found mainly in genes SDH1, SDH3, MDH1 and the transcription factor HAP4, regarding the number of single nucleotide polymorphisms and the gene copy-number profile. Real-time PCR was used to study gene expression of 10 selected genes involved in the metabolic pathway of succinic acid production. Results show that for the non-producing strain, higher expression of genes SDH1, SDH2, ADH1, ADH3, IDH1 and HAP4 was detected, together with lower expression of ADR1 transcription factor, in comparison with the best producer strain. This is the first study showing the capacity of natural yeast isolates to produce high amounts of succinic acid, together with the understanding of the key factors associated, giving clues for strain improvement.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFranco-Duarte, R., Bessa, D., Gonçalves, F., Martins, R., Silva-Ferreira, A.C., Schuller, D., … Pais, C. (2017). Genomic and transcriptomic analysis of Saccharomyces cerevisiae isolates with focus in succinic acid production. FEMS Yeast Research, 17(6), fox057pt_PT
dc.identifier.doi10.1093/femsyr/fox057pt_PT
dc.identifier.eid85037599777
dc.identifier.eissn1567-1364
dc.identifier.issn1567-1356
dc.identifier.pmid28910984
dc.identifier.urihttp://hdl.handle.net/10400.14/25997
dc.identifier.wos000412838700004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationNORTE-01-0145-FEDER-000009
dc.relationBioTRANSformation of by-products from fruit and vegetable processing industry into valuable BIOproducts
dc.subjectSuccinic acidpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectNatural isolatespt_PT
dc.subjectGenomicspt_PT
dc.subjectTranscriptomicspt_PT
dc.titleGenomic and transcriptomic analysis of Saccharomyces cerevisiae isolates with focus in succinic acid productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioTRANSformation of by-products from fruit and vegetable processing industry into valuable BIOproducts
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F04050%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/289603/EU
oaire.citation.issue6
oaire.citation.titleFEMS Yeast Researchpt_PT
oaire.citation.volume17
oaire.fundingStream5876
oaire.fundingStreamFP7
person.familyNameFranco-Duarte
person.familyNameMartins
person.familyNameSilva Ferreira
person.familyNameSampaio
person.givenNameRicardo
person.givenNameRosa
person.givenNameAntonio Cesar
person.givenNamePaula
person.identifier293798
person.identifier452904
person.identifier.ciencia-idE318-BC33-B2A0
person.identifier.ciencia-id051A-5A02-E227
person.identifier.ciencia-id931A-48DA-23A8
person.identifier.ciencia-id3C1F-E878-1307
person.identifier.orcid0000-0002-2333-6127
person.identifier.orcid0000-0003-1842-4840
person.identifier.orcid0000-0002-1188-1021
person.identifier.orcid0000-0002-1415-4428
person.identifier.ridI-6594-2015
person.identifier.ridI-7566-2015
person.identifier.scopus-author-id16028614100
person.identifier.scopus-author-id56275265400
person.identifier.scopus-author-id55953380500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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