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Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance

2015Journal article Restricted access
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dc.contributor.authorYan, Le-Ping
dc.contributor.authorSilva-Correia, Joana
dc.contributor.authorOliveira, Mariana B.
dc.contributor.authorVilela, Carlos
dc.contributor.authorPereira, Hélder
dc.contributor.authorSousa, Rui A.
dc.contributor.authorMano, João F.
dc.contributor.authorOliveira, Ana L.
dc.contributor.authorOliveira, Joaquim M.
dc.contributor.authorReis, Rui L.
dc.date.accessioned2016-04-21T14:26:45Z
dc.date.available2016-04-21T14:26:45Z
dc.date.issued2015
dc.description.abstractNovel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (silk-nanoCaP) layer for osteochondral defect (OCD) regeneration, were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the silk-nanoCaP layer. The scaffold presented compressive moduli of 0.4 MPa in the wet state. Rabbit bone marrow mesenchymal stromal cells (RBMSCs) were cultured on the scaffolds, and good adhesion and proliferation were observed. The silk-nanoCaP layer showed a higher alkaline phosphatase level than the silk layer in osteogenic conditions. Subcutaneous implantation in rabbits demonstrated weak inflammation. In a rabbit knee critical size OCD model, the scaffolds firmly integrated into the host tissue. Histological and immunohistochemical analysis showed that collagen II positive cartilage and glycosaminoglycan regeneration presented in the silk layer, and de novo bone ingrowths and vessel formation were observed in the silk-nanoCaP layer. These bilayered scaffolds can therefore be promising candidates for OCD regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationYAN, Le-Ping; SILVA-CORREIA, Joana; OLIVEIRA, Mariana B.; VILELA, Carlos; PEREIRA, Hélder; SOUSA, Rui A.; MANO, João F.; OLIVEIRA, Ana L.; OLIVEIRA, Joaquim M.; REIS, Rui L. - Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance. Acta Biomaterialia. ISSN 1742-7061. Vol.12 (2015), p. 227–241pt_PT
dc.identifier.doi10.1016/j.actbio.2014.10.021
dc.identifier.eid84924991609
dc.identifier.eissn1878-7568
dc.identifier.issn1742-7061
dc.identifier.pmid25449920
dc.identifier.urihttp://hdl.handle.net/10400.14/19901
dc.identifier.wos000348686100024
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationREGPOT-CT2012-316331-POLARIS
dc.relationTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
dc.relationNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
dc.subjectBilayered scaffoldpt_PT
dc.subjectSilk fibroinpt_PT
dc.subjectCalcium phosphatept_PT
dc.subjectOsteochondral regenerationpt_PT
dc.subjectNanocompositept_PT
dc.titleBilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
oaire.awardTitleNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM%2F105703%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM-BPC%2F115977%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F64717%2F2009/PT
oaire.citation.endPage241
oaire.citation.startPage227
oaire.citation.titleActa Biomaterialia
oaire.citation.volume12
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
person.familyNameYan
person.familyNameSilva Correia
person.familyNameOliveira
person.familyNamevilela
person.familyNamePereira
person.familyNameSousa
person.familyNameMano
person.familyNameOliveira
person.familyNameReis
person.givenNameLeping
person.givenNameJoana
person.givenNameMariana
person.givenNamecarlos
person.givenNameHelder
person.givenNameRui
person.givenNameJoão
person.givenNameJoaquim Miguel
person.givenNameRui L.
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person.identifier.scopus-author-id37032413400
person.identifier.scopus-author-id51161893400
person.identifier.scopus-author-id7102960061
person.identifier.scopus-author-id26643558900
person.identifier.scopus-author-id57202066972
person.identifier.scopus-author-id56861715700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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