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Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

2004Journal article Open access
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dc.contributor.authorSilva, Joana
dc.contributor.authorCarvalho, Ana Sofia
dc.contributor.authorVotorino, Rui
dc.contributor.authorDomingues, Pedro
dc.contributor.authorTeixeira, Paula
dc.contributor.authorGibbs, paul
dc.date.accessioned2011-10-21T14:08:22Z
dc.date.available2011-10-21T14:08:22Z
dc.date.issued2004
dc.description.abstractProtein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.por
dc.identifier.citation"Protein and peptide letters" . ISSN 0929-866511:2 (2004) 189-194por
dc.identifier.urihttp://hdl.handle.net/10400.14/6688
dc.language.isoengpor
dc.publisherBentham Science Publishers Ltd.por
dc.relation.publisherversionhttp://www.benthamscience.com/ppl/por
dc.subjectLactobacillus bulgaricuspor
dc.subjectProtein extractionpor
dc.subject2-DE electrophoresispor
dc.subjectStainingpor
dc.subjectSensitivitypor
dc.titleProtein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gelspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameSilva
person.familyNameTeixeira
person.familyNameGibbs
person.givenNameJoana
person.givenNamePaula
person.givenNamePaul
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-6580-4363
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42069f79-2050-47f2-a42b-fab5c301e9a3
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublication.latestForDiscovery218d85c8-40f4-42c0-9a47-4e8ad9f72a96

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