Logo do repositório
 
Miniatura indisponível
Publicação

Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

Utilize este identificador para referenciar este registo.

Orientador(es)

Resumo(s)

Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.

Descrição

Palavras-chave

Lactobacillus bulgaricus Protein extraction 2-DE electrophoresis Staining Sensitivity

Contexto Educativo

Citação

"Protein and peptide letters" . ISSN 0929-866511:2 (2004) 189-194

Projetos de investigação

Unidades organizacionais

Fascículo

Editora

Bentham Science Publishers Ltd.

Licença CC