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Applications of optical DNA mapping in microbiology

dc.contributor.authorBogas, Diana
dc.contributor.authorNyberg, Lena
dc.contributor.authorPacheco, Rui
dc.contributor.authorAzevedo, Nuno F.
dc.contributor.authorBeech, Jason P.
dc.contributor.authorGomila, Margarita
dc.contributor.authorLalucat, Jorge
dc.contributor.authorManaia, Célia M.
dc.contributor.authorNunes, Olga C.
dc.contributor.authorTegenfeldt, Jonas O.
dc.contributor.authorWesterlund, Fredrik
dc.date.accessioned2017-12-18T17:48:08Z
dc.date.available2017-12-18T17:48:08Z
dc.date.issued2017
dc.description.abstractOptical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBogas, D., Nyberg, L., Pacheco, R., Azevedo, N. F., Beech, J. P., Gomila, M., . . . Westerlund, F. (2017). Applications of optical DNA mapping in microbiology. Biotechniques, 62(6), 255-267pt_PT
dc.identifier.doi10.2144/000114555pt_PT
dc.identifier.eid85020897312
dc.identifier.eissn1940-9818
dc.identifier.issn0736-6205
dc.identifier.pmid28625155
dc.identifier.urihttp://hdl.handle.net/10400.14/23712
dc.identifier.wos000403563300004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFuture Medicine
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleApplications of optical DNA mapping in microbiologypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage267
oaire.citation.issue6
oaire.citation.startPage255pt_PT
oaire.citation.titleBioTechniquespt_PT
oaire.citation.volume62pt_PT
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person.familyNameAzevedo
person.familyNameBeech
person.familyNameGomila
person.familyNameManaia
person.familyNameNunes
person.givenNameDiana
person.givenNameNuno
person.givenNameJason
person.givenNameMargarita
person.givenNameCélia
person.givenNameOlga
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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