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Response surface evaluation of microwave-assisted extraction conditions for Lycium barbarum bioactive compounds

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Abstract(s)

The effects of microwave-assisted extraction (MAE) on the qualitative and quantitative composition of extracted bioactive phytochemicals are strongly dependent on specific operational conditions. The impact of temperature, extraction time, and solvent dielectric properties on theMAE of antioxidant compounds fromgoji berry is hereby reported and discussed. Results were evaluated in terms of antioxidant capacity (ascertained by ABTS and ORAC assays), complemented with quantification of total phenolics content (TPC), and characterization of extracts through HPLC. Furthermore, the effect of storage temperature was also studied. Results revealed that temperature and solvent properties exert a significant effect on TPC and ORAC assays, whereas irradiation time plays a key role in the preparation of extracts to be evaluated through ABTS. The effect of solvent properties is tentatively explained considering the dielectric properties ofmethanol andwater,whereas the observed effect of temperature could be explained by its influence on the solubility of phenolic compounds. Industrial Relevance: A careful selection of operational conditions duringMAE, designed for a specific antioxidant assay, allows an increase in the bioactivity of the resultant extracts,which may become important in terms of industrial applications.

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Antioxidant capacity Goji berry MAE Response surface methodology Solvent dielectric properties Temperature

Citation

MENDES, Marta; CARVALHO, Ana P.; MAGALHÃES, Júlia M.C.S.; MOREIRA, Manuela; GUIDO, Luís; GOMES, Ana M. P.; DELERUE-MATOS, Cristina - Response surface evaluation of microwave-assisted extraction conditions for Lycium barbarum bioactive compounds. Innovative Food Science and Emerging Technologies. ISSN 1466-8564. Vol. 33 (2016), p. 319-326 http://dx.doi.org/10.1016/j.ifset.2015.12.025

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