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Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva

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dc.contributor.authorFerreira, Francisca T.S.M.
dc.contributor.authorMesquita, Raquel
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2020-06-15T08:47:13Z
dc.date.available2022-11-01T01:30:21Z
dc.date.issued2020
dc.description.abstractIn this work, two different microfluidic paper-based analytical devices (μPADs) were developed for the quantification of nitrite and nitrate in human saliva samples, in order to aid in the diagnosis of some diseases and health conditions associated with these ions. The development of these nitrite and nitrate μPADs involved several studies to optimize their design and construction, including an interference assessment and stability studies. These μPADs allowed a nitrite determination in a range of 5–250 μM with limits of detection and quantification of 0.05 μM and 0.17 μM, respectively, and a nitrate determination in the range 0.2–1.2 mM with limits of detection and quantification of 0.08 mM and 0.27 mM, respectively. As for the stability, both of the μPADs were stable when stored in vacuum at 4 °C (the nitrite μPAD for at least 60 days and the nitrate μPAD for at least of 14 days) and, after the sample placement, the nitrite and nitrate μPADs could be scanned within the first 4 and 2 h, respectively. The nitrite μPAD measurements were compared with the ones obtained from the standard colorimetric method and there were no statistically significant differences between these two methods. To evaluate the accuracy of nitrate μPAD measurements, 4 certified water samples were used and recovery studies using saliva samples were performed.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationFerreira, F.T.S.M., Mesquita, R.B.R., Rangel, A.O.S.S. (2020). Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva. Talanta, 219, art.n.º 121183pt_PT
dc.identifier.doi10.1016/j.talanta.2020.121183pt_PT
dc.identifier.eid85085842514
dc.identifier.issn0039-9140
dc.identifier.pmid32887101
dc.identifier.urihttp://hdl.handle.net/10400.14/30713
dc.identifier.wos000568750000004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectNitrite determinationpt_PT
dc.subjectNitrate reductionpt_PT
dc.subjectSaliva analysispt_PT
dc.subjectPoint-of-carept_PT
dc.subjectDisposable devicespt_PT
dc.titleNovel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human salivapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/Multi/50016/2019
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume219pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMesquita
person.familyNameSantos Silva Rangel
person.givenNameRaquel
person.givenNameAntónio Osmaro
person.identifierJ-4630-2013
person.identifier.ciencia-idEB1D-DEE3-0B60
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0001-7745-1860
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridJ-4630-2013
person.identifier.scopus-author-id7005556626
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf89887f1-4d1b-438b-8f03-27d9bda1de7b
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscoveryf89887f1-4d1b-438b-8f03-27d9bda1de7b
relation.isProjectOfPublication1f76a7db-2520-482f-b56b-7e01ea36dd1f
relation.isProjectOfPublication.latestForDiscovery1f76a7db-2520-482f-b56b-7e01ea36dd1f

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