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Dissemination of metallo-β-lactamase-producing pseudomonas aeruginosa in Serbian Hospital settings: expansion of ST235 and ST654 clones

2023-01-12Journal article Open access
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dc.contributor.authorKabic, Jovana
dc.contributor.authorFortunato, Gianuario
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorKekic, Dusan
dc.contributor.authorJovicevic, Milos
dc.contributor.authorPesovic, Jovan
dc.contributor.authorRanin, Lazar
dc.contributor.authorOpavski, Natasa
dc.contributor.authorManaia, Célia M
dc.contributor.authorGajic, Ina
dc.date.accessioned2023-01-24T15:11:53Z
dc.date.available2023-01-24T15:11:53Z
dc.date.issued2023-01-12
dc.description.abstractThis nationwide study aimed to investigate the molecular characteristics of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in Serbia, underlying resistance mechanisms, the genetic context of detected MBL genes, and the clonal relationship between isolates harboring genes-encoding MBL. Overall, 320/5334 isolates collected from 2018 to 2021 were identified as P. aeruginosa. Carbapenem-resistant P. aeruginosa (CRPA) were screened for the presence of bla VIM , bla IMP , and bla NDM , genes whereas MBL-positive isolates were tested for the presence of the bla CTX-M-2 , bla PER , bla TEM , bla SHV , bla VEB , and bla GES . Multilocus sequence typing and phylogenomic analysis were performed for P. aeruginosa-producing MBL. The majority of the P. aeruginosa isolates were recovered from the lower respiratory tract (n = 120; 37.5%) and wound specimens (n = 108; 33.75%). CRPA isolates accounted for 43.1% (n = 138) of the tested isolates, 31 out of them being bla NDM-1 -positive (22.5%). The colistin resistance rate was 0.3%. MLST analysis revealed the occurrence of ST235 (n = 25) and ST654 (n = 6), mostly confined to Serbia. The distribution of beta-lactamase-encoding genes in these isolates suggested clonal dissemination and possible recombination: ST235/ bla NDM-1 , ST235/ bla NDM-1 / bla PER-1 , ST654/ bla NDM-1 , ST654/ bla NDM-1 / bla PER-1 , and ST654/ bla NDM-1 / bla GES-5 . High-risk clones ST235 and ST654 identified for the first time in Serbia, are important vectors of acquired MBL and ESBL and their associated multidrug resistance phenotypes represent a cause for considerable concern.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms24021519pt_PT
dc.identifier.eid85146782852
dc.identifier.issn1661-6596
dc.identifier.pmcPMC9863560
dc.identifier.pmid36675030
dc.identifier.urihttp://hdl.handle.net/10400.14/39987
dc.identifier.wos000914294400001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHigh-risk clonespt_PT
dc.subjectCarbapenem-resistant Pseudomonas aeruginosapt_PT
dc.subjectblaNDMpt_PT
dc.subjectblaPERpt_PT
dc.subjectblaGESpt_PT
dc.subjectMLSTpt_PT
dc.subjectWGSpt_PT
dc.titleDissemination of metallo-β-lactamase-producing pseudomonas aeruginosa in Serbian Hospital settings: expansion of ST235 and ST654 clonespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume24pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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