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Evaluation of enhanced haemolysis agar for detection of Listeria spp. and L. monocytogenes from production lines of fresh to cold-smoked fish

2001Journal article Open access
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dc.contributor.authorVaz-Velho, Manuela
dc.contributor.authorDuarte, Gabriela
dc.contributor.authorGibbs, Paul
dc.date.accessioned2011-10-22T16:24:11Z
dc.date.available2011-10-22T16:24:11Z
dc.date.issued2001
dc.description.abstractEnhanced haemolysis agar (EHA) was compared to the two conventional Listeria isolation agars Oxford and PALCAM for its ability to detect Listeria spp. from production lines of fresh to cold-smoked fish. The ability of EHA for distinguishing L. monocytogenes colonies from other Listeria spp. was also evaluated. A total of 243 fish and environmental samples were analysed. Overall, 42 samples were found to contain Listeria spp. Only 34 samples were positive simultaneously by the three plating media. Two samples considered to be negative by the two conventional agars were found to be positive after isolation on EHA. All three selective agars were shown to be less effective in recovering Listeria spp. after primary enrichment in half-Fraser broth, compared to secondary enrichment in Fraser broth after 24 and 48 h. From 79 Listeria but presumptive negative L. monocytogenes colonies, EHA identified correctly 76 Listeria spp. and presented three false-negative results_three colonies further identified as L. monocytogenes but showing no noticeable haemolysis on EHA. Twenty-three of the thirty-three L. monocytogenes presumptive positive colonies, were confirmed positive and ten were identified as L. seeligeri. Despite its ability of distinguishing L. monocytogenes from the other Listeria spp., unless it is produced as a commercial medium, EHA cannot be an alternative to time-consuming classical identification because the preparation of this medium is both time and labour intensive.por
dc.identifier.citationVAZ-VELHO, Manuela ; DUARTE, Gabriela ; GIBBS, Paul - Evaluation of enhanced haemolysis agar for detection of Listeria spp. and L. monocytogenes from production lines of fresh to cold-smoked fish. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 46, n.º 2 (2001), p. 157-163por
dc.identifier.doi10.1016/S0167-7012(01)00264-0
dc.identifier.urihttp://hdl.handle.net/10400.14/6840
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectHaemolysispor
dc.subjectListeria monocytogenespor
dc.subjectListeria selective plating mediapor
dc.subjectListeria spp.por
dc.titleEvaluation of enhanced haemolysis agar for detection of Listeria spp. and L. monocytogenes from production lines of fresh to cold-smoked fishpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameVaz Velho
person.familyNameGibbs
person.givenNameManuela
person.givenNamePaul
person.identifier.ciencia-idC719-383D-5548
person.identifier.orcid0000-0003-4825-2058
person.identifier.orcid0000-0002-6580-4363
person.identifier.ridK-3058-2014
person.identifier.scopus-author-id55999738500
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationceca7b30-6960-4ad2-bb0c-675fcca89f86
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublication.latestForDiscovery218d85c8-40f4-42c0-9a47-4e8ad9f72a96

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