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SE-HPLC as a tool to guide the production of novel peptide fractions for textile application

dc.contributor.authorBonifácio-Lopes, Teresa
dc.contributor.authorAfonso, Tiago Barros
dc.contributor.authorCoscueta, Ezequiel
dc.contributor.authorCosta, Eduardo M.
dc.contributor.authorPintado, Manuela
dc.date.accessioned2024-07-12T11:39:45Z
dc.date.available2024-07-12T11:39:45Z
dc.date.issued2024-03-25
dc.description.abstractIntroduction: Textile industry needs more bio centered solutions - the industry needs to adopt circular economy principles to repurpose its by-products; There is a need to re-utilize leather by-products and to develop biobased products1,2; SE-HPLC aids in the selection and production of peptide-rich solutions, these solutions are intended for use as functional coatings in textiles. This methodology helps optimize the formulation of coatings for desired functional properties. Objectives: Utilize SE-HPLC to gather fundamental data on the hydrolysis of protein-rich byproducts. Select and produce peptide-rich solutions suitable for functional textiles coatings. Evaluate different enzymes and hydrolysis conditions using SE-HPLC. Repurpose protein-rich leather by-products effectively using SE-HPLC guided strategies. Conclusions: Alcalase 10% 6 hours had higher hydrolysis degree (70.12%) than all the other enzymes percentage/time of hydrolysis Between time 0 and pre-final, molecules MW lower than 0.2 kDa consistently have a higher area. Conducting an enzyme inactivation (2 hours/90ºC) might result in molecule aggregation, leading to an increase in molecules with MW exceeding 50 kDa. Utilizing this technique facilitates the identification of the optimal time/enzyme concentration ratio. It enables industries to repurpose waste effectively while developing new bio-based products through hydrolysis. Specifically, it aids in the production of functional coatings, offering a sustainable solution for waste utilization and product innovation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/45751
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.titleSE-HPLC as a tool to guide the production of novel peptide fractions for textile applicationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortugalpt_PT
oaire.citation.titleAnalítica 2024: 11th Meeting of the SPQ Analytical Chemistry Divisionpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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