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EPS production by bacteria from high salinity wastewater.

dc.contributor.authorPaulo, Ana M.S.
dc.contributor.authorAmorim, Catarina L.
dc.contributor.authorCastro, Paula M.L.
dc.date.accessioned2018-06-12T10:26:57Z
dc.date.available2018-06-12T10:26:57Z
dc.date.issued2017-12-07
dc.description.abstractAerobic granular sludge (AGS) is a promising technology for treating industrial wastewater, possessing higher biomass retention and tolerance to toxic substrates than conventional activated sludge systems. The presence of extracellular polymeric substances (EPS) in the aerobic granules structure increases the bacterial protection towards stress conditions and the stability of granules. Several industrial wastewaters contain high salt concentrations in their composition, which can inhibit the bacteria responsible for nutrients removal in the AGS process [1]. A novel strategy to increase the robustness of the system to high salinity is to bioaugment the aerobic granules with halotolerant bacteria with ability to produce EPS. Using a robust AGS process, extra value can be obtained from the wastewater since the EPS from the biomass can be recovered and used as new biomaterials in different applications. The aim of the present study is to investigate the feasibility of EPS production by halotolerant bacteria isolated from high salinity wastewater collected in a fish canning industry. Bacterial isolates obtained from the saline water were grouped according to species similarity, based on RAPD profiles. Isolates displaying unique RAPD profiles were subsequently identified by 16S rRNA gene sequencing analysis. The potential for EPS production by isolates presenting a sticky growth on agar plates is currently being evaluated. The content in proteins, humic acids and carbohydrates of the extracted EPS is quantified using reference methods. The obtained isolates are closely related to bacterial strains from the Acinetobacter, Psychrobacter, Arthrobacter, Bacillus, Exiguobacterium and Kocuria genera. The isolated halotolerant bacteria present different ability to produce EPS. The most promising EPS producers will be used for bioaugmentation of an AGS process treating high salinity wastewater. Valorization of the wastewater through EPS recovery from the AGS is in line with the circular economy concept.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/25048
dc.language.isoengpt_PT
dc.relationPOCI-01-0145-FEDER-016403 - MULTIBIOREFINERYpt_PT
dc.subjectEPSpt_PT
dc.subjectHigh salinity wastewaterpt_PT
dc.titleEPS production by bacteria from high salinity wastewater.pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F96481%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleCongress of Microbiology and Biotechnology 2017 - MICROBIOTEC’17pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNameCastro
person.givenNamePaula
person.identifier2013444
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscoveryf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isProjectOfPublication3d55bd0a-4633-46da-bb6e-8d4c13a01f04
relation.isProjectOfPublication3fe5970e-de39-42f7-a29d-18521b591a09
relation.isProjectOfPublication.latestForDiscovery3fe5970e-de39-42f7-a29d-18521b591a09

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