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Diversity and antibiotic resistance in Pseudomonas spp. from drinking water

2012Journal article Restricted access
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dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2013-01-28T12:19:44Z
dc.date.available2013-01-28T12:19:44Z
dc.date.issued2012
dc.description.abstractPseudomonas spp. are common inhabitants of aquatic environments, including drinking water. Multiantibiotic resistance in clinical isolates of P. aeruginosa is widely reported and deeply characterized. However, the information regarding other species and environmental isolates of this genus is scant. This study was designed based on the hypothesis that members of the genus Pseudomonas given their high prevalence, wide distribution in waters and genetic plasticity can be important reservoirs of antibiotic resistance in drinking water. With this aim, the diversity and antibiotic resistance phenotypes of Pseudomonas isolated from different drinking water sources were evaluated. The genotypic diversity analyses were based on six housekeeping genes (16S rRNA, rpoD, rpoB, gyrB, recA and ITS) and on pulsed field gel electrophoresis. Susceptibility to 21 antibiotics of eight classes was tested using the ATB PSE EU (08) and disk diffusion methods. Pseudomonas spp. were isolated from 14 of the 32 sampled sites. A total of 55 non-repetitive isolates were affiliated to twenty species. Although the same species were isolated from different sampling sites, identical genotypes were never observed in distinct types of water (water treatment plant/distribution system, tap water, cup fillers, biofilm, and mineral water). In general, the prevalence of antibiotic resistance was low and often the resistance patterns were related with the species and/or the strain genotype. Resistance to ticarcillin, ticarcillin with clavulanic acid, fosfomycin and cotrimoxazol were the most prevalent (69–84%). No resistance to piperacillin, levofloxacin, ciprofloxacin, tetracycline, gentamicin, tobramycin, amikacin, imipenem or meropenem was observed. This study demonstrates that Pseudomonas spp. are not so widespread in drinking water as commonly assumed. Nevertheless, it suggests that water Pseudomonas can spread acquired antibiotic resistance, preferentially via vertical transmission.por
dc.description.versionN/A
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationVAZ-MOREIRA, Ivone; NUNES, Olga C.; MANAIA, C. M. - Diversity and antibiotic resistance in Pseudomonas spp. from drinking water. Science of the Total Environment. ISSN 0048-9697. Vol. 426, (2012), p. 366–374por
dc.identifier.doi10.1016/j.scitotenv.2012.03.046
dc.identifier.eissn1879-1026
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.14/9978
dc.identifier.wosWOS:000304795300042
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectPseudomonaspor
dc.subjectDrinking waterpor
dc.subjectAntibiotic resistancepor
dc.subjectrpoDpor
dc.subjectgyrBpor
dc.subjectrpoBpor
dc.titleDiversity and antibiotic resistance in Pseudomonas spp. from drinking waterpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAMB%2F70825%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F27978%2F2006/PT
oaire.citation.endPage374
oaire.citation.startPage366
oaire.citation.titleScience of the Total Environment
oaire.citation.volume426
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameVaz-Moreira
person.familyNameNunes
person.familyNameManaia
person.givenNameIvone
person.givenNameOlga
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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