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Caseinolytic activity of fruit extract from Opuntia ficus-indica on bovine, caprine, and ovine Sodium Caseinates

2001Journal article Open access
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dc.contributor.authorPintado, Ana I. E.
dc.contributor.authorMacedo, Angela C.
dc.contributor.authorTeixeira, Grimaneza
dc.contributor.authorPais, Salomé
dc.contributor.authorClemente, Alda
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-22T22:46:32Z
dc.date.available2011-10-22T22:46:32Z
dc.date.issued2001
dc.description.abstractThe rates and extents of hydrolysis of RS- and â-caseins from bovine, caprine, and ovine sodium caseinates produced by an enzymatic extract of the fruit of Opuntia ficusindica, (L.) Miller were evaluated and compared with those produced by a commercial animal rennet. A mechanistic model based on a pseudo-first-order enzymatic reaction, in the presence of first-order deactivation of the enzyme, was postulated and successfully fitted to the experimental data. The animal rennet exhibited higher enzymatic efficiency than the fruit extract, irrespective of the source (i.e., bovine, caprine, or ovine) and the type (i.e., RS- or â-casein) of substrate. The enzymatic efficiency (kcat/Km) for RS-casein ranged from 72 to 220 and from 43 to 65 L g-1 h-1, and for â-casein from 242 to 742 and from 55 to 164 L g-1 h-1, for the animal rennet and the enzymatic extract of O. ficus-indica, respectively. Finally, it was observed that â-casein from caprine and ovine caseinates was degraded by O. ficus-indica faster than its RS counterpart, but the reverse was observed for bovine caseinate.por
dc.identifier.citationPINTADO, Ana I...[et al.] - Caseinolytic Activity of Fruit Extract from Opuntia ficus-indica on Bovine, Caprine, and Ovine Sodium Caseinates. Biotechnology Progress. ISSN 8756-7938. Vol. 17, n.º 4 (2001), p. 643-646por
dc.identifier.doi10.1021/bp0100474
dc.identifier.urihttp://hdl.handle.net/10400.14/6900
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Society e American Institute of Chemical Engineerspor
dc.titleCaseinolytic activity of fruit extract from Opuntia ficus-indica on bovine, caprine, and ovine Sodium Caseinatespor
dc.typejournal article
dspace.entity.typePublication
person.familyNamePintado
person.familyNameMacedo
person.familyNameMalcata
person.givenNameAna
person.givenNameAngela
person.givenNameFrancisco
person.identifier.ciencia-id871B-4017-FC07
person.identifier.ciencia-idE116-EC15-79C5
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-4931-708X
person.identifier.orcid0000-0002-6854-9855
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id6602847645
person.identifier.scopus-author-id7101906752
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationcf43b696-b273-4051-bb79-3a9f8fb63940
relation.isAuthorOfPublication6601534a-2130-4525-bf92-604e4831bb9a
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverycf43b696-b273-4051-bb79-3a9f8fb63940

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