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Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production

2010Journal article Open access
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dc.contributor.authorSampaio, Pedro N.
dc.contributor.authorCalado, Cecília R. C.
dc.contributor.authorSousa, Lisete
dc.contributor.authorBressler, David C.
dc.contributor.authorPais, Maria Salomé
dc.contributor.authorFonseca, Luís Pina
dc.date.accessioned2011-10-18T13:59:27Z
dc.date.available2011-10-18T13:59:27Z
dc.date.issued2010
dc.description.abstractThe optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSAMPAIO, Pedro N. [et al.] - Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. European food research and technology [Em linha]. 231:2 (2010) 339-346. Disponível na Internet: http://www.springerlink.com/content/ar5871t50476k014 . ISSN 1438-2385.por
dc.identifier.doi10.1007/s00217-010-1281-z
dc.identifier.eissn1438-2385
dc.identifier.issn1438-2385
dc.identifier.urihttp://hdl.handle.net/10400.14/6422
dc.identifier.wosWOS:000277639000019
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer-Verlagpor
dc.relationPROCESSOS INTEGRADOS DE PRODUÇÃO E RECUPERAÇÃO IN SITU DE CIPROZINAS POR LEVEDURAS RECOMBINANTES
dc.subjectCulture media compositionpor
dc.subjectCyprosin Bpor
dc.subjectFermentationpor
dc.subjectResponse surface methodologypor
dc.subjectSaccharomyces cerevisiaepor
dc.titleOptimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese productionpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePROCESSOS INTEGRADOS DE PRODUÇÃO E RECUPERAÇÃO IN SITU DE CIPROZINAS POR LEVEDURAS RECOMBINANTES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F8780%2F2002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAT%2F64353%2F2006/PT
oaire.citation.endPage346por
oaire.citation.issue2
oaire.citation.startPage339por
oaire.citation.titleEuropean Food Research and Technologypor
oaire.citation.volume231
oaire.fundingStream3599-PPCDT
person.familyNameSampaio
person.familyNameCalado
person.familyNameSousa
person.familyNamePais
person.familyNameP. Fonseca
person.givenNamePedro
person.givenNameCecília
person.givenNameLisete
person.givenNameMaria Salomé
person.givenNameLuis
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id6518-7145-2F61
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2114-720X
person.identifier.orcid0000-0002-3068-5851
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridM-1842-2015
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id24802739400
person.identifier.scopus-author-id7103096414
person.identifier.scopus-author-id55916288400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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