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Abstract(s)
The phenotypic and genotypic characteristics of four Bordetella hinzii-like strains from human
respiratory specimens and representing nrdA gene sequence based genogroups 3, 14 and 15 were
examined. In a 16S rRNA gene sequence based phylogenetic tree, the four strains consistently
formed a single coherent lineage but their assignment to the genus Bordetella was equivocal. The
respiratory quinone, polar lipid and fatty acid profiles generally conformed to those of species of the
genus Bordetella and were characterized by the presence of ubiquinone 8, of
phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several aminolipids, and
of high percentages of C16 : 0, cyclo-C17 : 0 and summed feature 2, as major chemotaxonomic
marker molecules, respectively. The DNA G+C content was about 66 mol%, which corresponded
with that of the high-percentage DNA G+C content genera of the family Alcaligenaceae including
the genus Bordetella. DNA–DNA hybridization experiments revealed the presence of three distinct
genomospecies and thus confirmed phenotypic differences as revealed by means of extensive
biochemical characterization.We therefore propose to formally classify Bordetella genogroups 3, 14
and 15 as Bordetella bronchialis sp. nov. (type strain LMG 28640T5AU3182T5CCUG 56828T),
Bordetella sputigena sp. nov. (type strain LMG 28641T5CCUG 56478T) and Bordetella flabilis
sp. nov. (type strain LMG 28642T5AU10664T5CCUG 56827T). In addition, we propose to
reclassify Achromobacter sediminum into the novel genus Verticia, as Verticia sediminum, gen. nov.,
comb. nov., on the basis of its unique phylogenetic position, its marine origin and its distinctive
phenotypic, fatty acid and polar lipid profile
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Citation
LOPES, Ana Rita; BECERRA-CASTRO, Cristina; VAZ-MOREIRA, Ivone; SILVA, M. Elisabete F.; NUNES, Olga C.; MANAIA, C. M. - Irrigation with Treated Wastewater: Potential Impacts on Microbial Function and Diversity in Agricultural Soils. Wastewater Reuse and Current Challenges. ISSN 1867-979x. Vol. 44 (2015), p. 105-128
Publisher
Microbiology Society