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Insights into chitosan antibioļ¬lm activity against methicillin-resistant Staphylococcus aureus

2017Journal article Restricted access
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dc.contributor.authorCosta, E. M.
dc.contributor.authorSilva, S.
dc.contributor.authorTavaria, F. K.
dc.contributor.authorPintado, M. M.
dc.date.accessioned2017-11-14T14:49:57Z
dc.date.available2017-11-14T14:49:57Z
dc.date.issued2017
dc.description.abstractAims: Chitosan is a natural compound that has been validated as a viable antimicrobial agent against Staphylococcus aureus. With this work we sought to evaluate the planktonic and sessile sensitivity of methicillin-resistant S. aureus to chitosan's activity and evaluate if methicillin-resistant S. aureus (MRSA) would be more or less sensitive to chitosan's activity than methicillin-sensitive S. aureus (MSSA). Methods and Results: A group comprised of reference strains and clinical multiresistant isolates of MSSA and MRSA were used. Methicilin resistance effect upon chitosan activity was assessed in planktonic setting and in different phases of sessile colonization, namely adhesion, biofilm formation and mature biofilm through biomass and metabolism inhibition. The results obtained showed that S. aureus methicillin resistance mechanism did not impair chitosan's activity as the highest bacterial susceptibility was registered for MRSA. Chitosan was highly effective in inhibiting MSSA and MRSA strains in both planktonic and sessile settings with biofilm inhibition percentages reaching as high as 90% for MRSA. Conclusions: Staphylococcus aureus methicillin resistance did not impair chitosan's antimicrobial and antibiofilm activities and MRSA and MSSA were inhibited both in planktonic and sessile settings at low concentrations with great efficacy. Significance and Impact of the Study: Considering the obtained results chitosan shows potential as an alternative for the control of biofilm-related recalcitrant MRSA infections.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, E. M., Silva, S., Tavaria, F., Pintado, M. E. (2017). Insights into chitosan antibiofilm activity against methicillin-resistant Staphylococcus aureus. Journal of Applied Microbiology, 122(6), 1547-1557pt_PT
dc.identifier.doi10.1111/jam.13457pt_PT
dc.identifier.eid85018260964
dc.identifier.eissn1365-2672
dc.identifier.issn1364-5072
dc.identifier.pmid28370752
dc.identifier.urihttp://hdl.handle.net/10400.14/23365
dc.identifier.wos000401538200011
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationTextile staining: Toward a greener and functional solution
dc.subjectAntibiofilm activitypt_PT
dc.subjectBiofilmspt_PT
dc.subjectChitosanpt_PT
dc.subjectMethicillin resistancept_PT
dc.subjectMRSApt_PT
dc.subjectMSSApt_PT
dc.titleInsights into chitosan antibioļ¬lm activity against methicillin-resistant Staphylococcus aureuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTextile staining: Toward a greener and functional solution
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBDE%2F103957%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F90867%2F2012/PT
oaire.citation.endPage1557
oaire.citation.issue6
oaire.citation.startPage1547
oaire.citation.titleJournal of Applied Microbiologypt_PT
oaire.citation.volume122
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameCosta
person.familyNameSilva
person.familyNameKekhasharĆŗ Tavaria
person.familyNamePintado
person.givenNameEduardo
person.givenNameSara
person.givenNameFreni
person.givenNameMaria Manuela
person.identifier1888852
person.identifier456608
person.identifier.ciencia-idB218-870B-98B5
person.identifier.ciencia-id8E18-0AFF-BF6D
person.identifier.ciencia-id1B15-5DAD-7B27
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-3121-4514
person.identifier.orcid0000-0001-8268-2137
person.identifier.orcid0000-0002-6273-6668
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id55249865700
person.identifier.scopus-author-id55158300800
person.identifier.scopus-author-id6602645613
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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