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Advisor(s)
Abstract(s)
Environmental fate assessment of chiral pharmaceuticals is an important issue
and little information is known about enantioselectivity in the environment. This kind of
information is important for regulamentation of pharmaceutical industry and chiral
switching
processes. Fluoxetine (FLX), an anti-depressant worldwide used, is a chiral
pharmaceutical prescribed in racemic form, and its main metabolite norfluoxetine (NFLX)
is also chiral. In this study, enantioselective degradation of rac-FLX and degradation of its
enantiomers separately, in a minimal salts medium inoculated by a bacterium consortium
was examined both at light and dark conditions. Theassays were performed in a shaker
at aerobic and ambient temperature conditions. The analytical method used was an
enantioselective HPLC-FD method using a vancomycin-based chiral stationary phase in
reversed mode to monitor enantiomers of FLX and NFLX. No degradation of enantiomers
of FLX in the abiotic controls was observed. In theall assays (R)-FLX was degraded faster
and totally until day 24th while (S)-FLX remained up to 20% of its initial concentration
until the end of the experiment (38 days). NFLX wasdetected in all biotic experiments.
Description
Keywords
biodegradation chiral pharmaceuticals
Citation
RIBEIRO, Ana R. [et al.] - Enantioselective Degradation of Enantiomers of Fluoxetine Followed by HPLC- FD. In 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Amsterdam, The Netherlands, 16-20 June 2013 – In HPLC 2013 - 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques: Book of Abstracts, (2013) p.297