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Silk-based anisotropical 3D biotextiles for bone regeneration

2017Journal article Open access
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dc.contributor.authorRibeiro, Viviana P.
dc.contributor.authorSilva-Correia, Joana
dc.contributor.authorNascimento, Ana I.
dc.contributor.authorMorais, Alain da Silva
dc.contributor.authorMarques, Alexandra P.
dc.contributor.authorRibeiro, Ana S.
dc.contributor.authorSilva, Carla J.
dc.contributor.authorBonifácio, Grata
dc.contributor.authorSousa, Rui A.
dc.contributor.authorOliveira, Joaquim M.
dc.contributor.authorOliveira, Ana L.
dc.contributor.authorReis, Rui L.
dc.date.accessioned2017-10-12T15:11:39Z
dc.date.available2020-01-15T01:30:53Z
dc.date.issued2017
dc.description.abstractBone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationRIBEIRO, V.P.; SILVA-CORREIA, J.; NASCIMENTO, A.I.; MORAIS, A. DA SILVA; MARQUES, A.P.; RIBEIRO, A.S.; SILVA, C.J.; BONIFÁCIO, G.; SOUSA, R.A.; OLIVEIRA, J. M.; OLIVEIRA, Ana L.; REIS, R.L. (2017). Silk-Based Anisotropical 3D Biotextiles for Bone Regeneration. Biomaterials, 123, 92-106pt_PT
dc.identifier.doi10.1016/j.biomaterials.2017.01.027pt_PT
dc.identifier.eid85012273606
dc.identifier.eissn1878-5905
dc.identifier.issn0142-9612
dc.identifier.pmid28161684
dc.identifier.urihttp://hdl.handle.net/10400.14/23052
dc.identifier.wos000395597800008
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationPD/59/2013
dc.relationBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
dc.relationTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectBiotextilept_PT
dc.subjectTextilept_PT
dc.subjectSilk Fibroinpt_PT
dc.subjectKnitted Spacer Fabricspt_PT
dc.subjectHuman Adipose-Derived Stem Cellspt_PT
dc.subjectCraniofacial Bone Tissue Engineeringpt_PT
dc.titleSilk-based anisotropical 3D biotextiles for bone regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
oaire.awardTitleTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F113806%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM%2F105703%2F2008/PT
oaire.citation.endPage106
oaire.citation.startPage92
oaire.citation.titleBiomaterialspt_PT
oaire.citation.volume123
oaire.fundingStreamOE
oaire.fundingStream5876-PPCDTI
person.familyNameRibeiro
person.familyNameSilva Correia
person.familyNameNascimento
person.familyNameda Silva Morais
person.familyNameMarques
person.familyNameSilva
person.familyNameSousa
person.familyNameOliveira
person.familyNameOliveira
person.familyNameReis
person.givenNameDr. Viviana
person.givenNameJoana
person.givenNameAna Isabel
person.givenNameAlain
person.givenNameAlexandra
person.givenNameCarla
person.givenNameRui
person.givenNameJoaquim Miguel
person.givenNameAna
person.givenNameRui L.
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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