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Advisor(s)
Abstract(s)
Fluoroquinolone (FQ) antibiotics are extensively
used both in human and veterinary medicine, and their
accumulation in the environment is causing an increasing
concern. In this study, the biodegradation of the three most
worldwide used FQs, namely ofloxacin, norfloxacin, and
ciprofloxacin, by the fluoroorganic-degrading strain Labrys
portucalensis F11 was assessed. Degradation occurred when
the FQs were supplied individually or as mixture in the culture
medium, in the presence of an easily degradable carbon
source. Consumption of individual FQs was achieved at
different extents depending on its initial concentration,
ranging from 0.8 to 30 μM. For the lowest concentration, total
uptake of each FQ was observed but stoichiometric fluoride
release was not achieved. Intermediate compounds were
detected and identified by LC-MS/MS with a quadrupole time
of flight detector analyzer. Biotransformation of FQs by L.
portucalensis mainly occurred through a cleavage of the piperazine ring and displacement of the fluorine substituent
allowing the formation of intermediates with less antibacterial
potency. FQ-degrading microorganisms could be useful for
application in bioaugmentation processes towards more
efficient removal of contaminants in wastewater treatment
plants.
Description
Keywords
Fluoroquinolones Labrys portucalensis F11 Biodegradation Metabolites
Citation
AMORIM, Catarina L... [et al.] - Biodegradation of ofloxacin, norfloxacin, and ciprofloxacin as single and mixed substrates by Labrys portucalensis F11. Applied Microbiology and Biotechnology. ISSN 1432-0614. Vol. 98, n.º 7 (2014), p. 3181-3190
Publisher
Springer