Logo do repositório
 
Publicação

Functional in-vitro evaluation of the non-specific effects of BCG vaccination in a randomised controlled clinical study

2022-05-12Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorWilkie, Morven
dc.contributor.authorTanner, Rachel
dc.contributor.authorWright, Daniel
dc.contributor.authorRamon, Raquel Lopez
dc.contributor.authorBeglov, Julia
dc.contributor.authorRiste, Michael
dc.contributor.authorMarshall, Julia L.
dc.contributor.authorHarris, Stephanie A.
dc.contributor.authorBettencourt, Paulo J. G.
dc.contributor.authorHamidi, Ali
dc.contributor.authorDiemen, Pauline M. van
dc.contributor.authorMoss, Paul
dc.contributor.authorSatti, Iman
dc.contributor.authorWyllie, David
dc.contributor.authorMcShane, Helen
dc.date.accessioned2022-05-26T13:01:44Z
dc.date.available2022-05-26T13:01:44Z
dc.date.issued2022-05-12
dc.description.abstractBacille Calmette-Guerin (BCG), the only currently licenced tuberculosis vaccine, may exert beneficial non-specific effects (NSE) in reducing infant mortality. We conducted a randomised controlled clinical study in healthy UK adults to evaluate potential NSE using functional in-vitro growth inhibition assays (GIAs) as a surrogate of protection from four bacteria implicated in infant mortality. Volunteers were randomised to receive BCG intradermally (n = 27) or to be unvaccinated (n = 8) and were followed up for 84 days; laboratory staff were blinded until completion of the final visit. Using GIAs based on peripheral blood mononuclear cells, we observed a significant reduction in the growth of the Gram-negative bacteria Escherichia coli and Klebsiella pneumonia following BCG vaccination, but no effect for the Gram-positive bacteria Staphylococcus aureus and Streptococcus agalactiae. There was a modest association between S. aureus nasal carriage and growth of S. aureus in the GIA. Our findings support a causal link between BCG vaccination and improved ability to control growth of heterologous bacteria. Unbiased assays such as GIAs are potentially useful tools for the assessment of non-specific as well as specific effects of TB vaccines. This study was funded by the Bill and Melinda Gates Foundation and registered with ClinicalTrials.gov (NCT02380508, 05/03/2015; completed).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-022-11748-xpt_PT
dc.identifier.eid85129918133
dc.identifier.issn2045-2322
dc.identifier.pmcPMC9096342
dc.identifier.pmid35552463
dc.identifier.urihttp://hdl.handle.net/10400.14/37739
dc.identifier.wos000795163100044
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleFunctional in-vitro evaluation of the non-specific effects of BCG vaccination in a randomised controlled clinical studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume12pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
43882801.pdf
Tamanho:
1.56 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

CIIS - Contribuições em Revistas Científicas / Contribution to Journals