Publication
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation
| dc.contributor.author | Emanuelsson, Maria A. E. | |
| dc.contributor.author | Osuna, M. Begoña | |
| dc.contributor.author | Jorge, Ruben M. Ferreira | |
| dc.contributor.author | Castro, Paula M. L. | |
| dc.date.accessioned | 2010-11-02T14:39:45Z | |
| dc.date.available | 2010-11-02T14:39:45Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM. | por |
| dc.description.version | info:eu-repo/semantics/publishedVersion | |
| dc.identifier.citation | EMANUELSSON, Maria A. E ...[et al.] - Isolation of a xanthobacter sp. degrading dichloromethane. Biodegradation. ISSN 1572-9729. Vol. 20, n.º 2 (2009), p. 235–244 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.14/3262 | |
| dc.language.iso | eng | por |
| dc.publisher | Springer Verlag | por |
| dc.subject | Dechlorination | por |
| dc.subject | Dichloromethane | por |
| dc.subject | DCM dehalogenase | por |
| dc.subject | Halogenated organic | por |
| dc.subject | Xanthobacter | por |
| dc.title | Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Castro | |
| person.givenName | Paula | |
| person.identifier | 2013444 | |
| person.identifier.ciencia-id | 7C1F-6C72-354A | |
| person.identifier.orcid | 0000-0001-8841-6606 | |
| person.identifier.rid | M-8241-2013 | |
| person.identifier.scopus-author-id | 7102781782 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | f2253992-dc8d-4042-9a0f-597ebcf0a1d6 | |
| relation.isAuthorOfPublication.latestForDiscovery | f2253992-dc8d-4042-9a0f-597ebcf0a1d6 |