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Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation

dc.contributor.authorEmanuelsson, Maria A. E.
dc.contributor.authorOsuna, M. Begoña
dc.contributor.authorJorge, Ruben M. Ferreira
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2010-11-02T14:39:45Z
dc.date.available2010-11-02T14:39:45Z
dc.date.issued2009
dc.description.abstractA bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationEMANUELSSON, Maria A. E ...[et al.] - Isolation of a xanthobacter sp. degrading dichloromethane. Biodegradation. ISSN 1572-9729. Vol. 20, n.º 2 (2009), p. 235–244por
dc.identifier.urihttp://hdl.handle.net/10400.14/3262
dc.language.isoengpor
dc.publisherSpringer Verlagpor
dc.subjectDechlorinationpor
dc.subjectDichloromethanepor
dc.subjectDCM dehalogenasepor
dc.subjectHalogenated organicpor
dc.subjectXanthobacterpor
dc.titleIsolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradationpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameCastro
person.givenNamePaula
person.identifier2013444
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id7102781782
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscoveryf2253992-dc8d-4042-9a0f-597ebcf0a1d6

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