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Characterization of the root transcriptome for iron and zinc homeostasis-related genes in indica rice (Oryza sativa L)

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dc.contributor.authorChandel, G.
dc.contributor.authorBanerjee, S.
dc.contributor.authorVasconcelos, M.
dc.contributor.authorGrusak, M. A.
dc.date.accessioned2011-08-01T16:11:31Z
dc.date.available2011-08-01T16:11:31Z
dc.date.issued2010
dc.description.abstractMicronutrient malnutrition is the most common form of nutrient deficiency among populations having a cereal-based diet. Rice is the staple food for one third of the world’s population but is poor source of iron and zinc content. We have characterized root transcriptome of diverse indica rice cultivars for expression of ten known metal homeostasis related genes in plants grown under controlled condition [with fe(III)-HEDTA iron source]. Fe/Zn contents of root and shoot tissues were also determined. Expression analysis showed expression of OsFRO2, OsZIP9, OsYSL3, OsIRT-1 and OsZip5 in most of the cultivars. The cDNA amplicons of OsFRO2 and OsZIP9 from different cultivars were analysed for sequence homology and several variation in their nucleotide sequences among rice genotypes. More than 94% sequence homology was observed for both the genes. We analysed the genomic region underlying these genes to obtain information about possible spatial localization based on overlapping ESTs and MPSS tags. Also, putative SNPs were identified within OsFRO2 and OsZIP9 genes that need to be validated. The sequence based information may be useful in further development of gene specific markers for screening and breeding of high iron and zinc lines.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationCHANDEL, G... [et al.] - Characterization of the root transcriptome for iron and zinc homeostasis-related genes in indica rice (Oryza sativa L). Journal of Plant Biochemistry and Biotechnology. ISSN 0971-7811. Vol. 19, n.º 2 (2010), p. 145-152por
dc.identifier.doi10.1007/BF03263334
dc.identifier.eissn0974-1275
dc.identifier.issn0971-7811
dc.identifier.urihttp://hdl.handle.net/10400.14/4899
dc.identifier.wosWOS:000281080200002
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherIndian Agricultural Research Institutepor
dc.subjectTranscriptomepor
dc.subjectMetal homeostasispor
dc.subjectCandidate genespor
dc.subjectSequence homologypor
dc.subjectIn-silicopor
dc.subjectRT-PCRpor
dc.titleCharacterization of the root transcriptome for iron and zinc homeostasis-related genes in indica rice (Oryza sativa L)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage152
oaire.citation.issue2
oaire.citation.startPage145
oaire.citation.titleJournal of Plant Biochemistry and Biotechnology
oaire.citation.volume19
person.familyNameVasconcelos
person.givenNameMarta
person.identifier74696
person.identifier.orcid0000-0002-5110-7006
person.identifier.ridI-8166-2013
person.identifier.scopus-author-id14627797100
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationdf60ebb4-3b71-4733-a9e3-bfa4687f5576
relation.isAuthorOfPublication.latestForDiscoverydf60ebb4-3b71-4733-a9e3-bfa4687f5576

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