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Screening of novel bioactive peptides from goat casein: in silico to in vitro validation

2022-03-01Artigo científico Acesso aberto
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dc.contributor.authorCoscueta, Ezequiel R.
dc.contributor.authorBatista, Patrícia
dc.contributor.authorGomes, José Erick Galindo
dc.contributor.authorSilva, Roberto da
dc.contributor.authorPintado, Maria Manuela
dc.date.accessioned2022-03-02T15:53:05Z
dc.date.available2022-03-02T15:53:05Z
dc.date.issued2022-03-01
dc.description.abstractFood-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms23052439pt_PT
dc.identifier.eid85125038672
dc.identifier.issn1661-6596
dc.identifier.pmcPMC8910560
dc.identifier.pmid35269581
dc.identifier.urihttp://hdl.handle.net/10400.14/36848
dc.identifier.wos000771500300001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectACE inhibitionpt_PT
dc.subjectAntihypertensive activitypt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectBioactive peptidept_PT
dc.subjectBioinformaticspt_PT
dc.subjectIn silico predictionpt_PT
dc.subjectORACpt_PT
dc.titleScreening of novel bioactive peptides from goat casein: in silico to in vitro validationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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