Publication
Carbodiimide modification enhances activity of pig pancreatic phospholipase A2
| dc.contributor.author | Ferreira, João Paulo M. | |
| dc.contributor.author | Sasisekharan, Ram | |
| dc.contributor.author | Louie, Otway | |
| dc.contributor.author | Langer, Robert | |
| dc.date.accessioned | 2011-09-13T14:44:12Z | |
| dc.date.available | 2011-09-13T14:44:12Z | |
| dc.date.issued | 1994 | |
| dc.description.abstract | Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in bovine phospholipase A2, pig iso-phospholipase A2, and pig phospholipase A2, respectively. With 1-oleoylglycerophosphocholine, the bovine enzyme lost 40% activity, but the pig enzyme was activated sevenfold. The modified pig enzyme showed pre-micellar activation on monomeric diheptanoyl-lecithin, and either reduced or increased activities on mixed micelles of bile salt with egg phosphatidylcholine, depending on the composition of the micelles. This activation is consistent with previous protein-engineering studies of pig pancreatic phospholipase A2. In this study, we present new information concerning the specificity and interfacial recognition behaviour of this enzyme in relation to this activation. | por |
| dc.identifier.citation | FERREIRA, João Paulo M... [et al.] - Carbodiimide modification enhances activity of pig pancreatic phospholipase A2. European Journal of Biochemistry. ISSN 1742-4658. Vol. 223, n.º 2 (1994), p. 611–616 | por |
| dc.identifier.doi | 10.1111/j.1432-1033.1994.tb19032.x | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/5484 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley Blackwell | por |
| dc.title | Carbodiimide modification enhances activity of pig pancreatic phospholipase A2 | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Ferreira | |
| person.givenName | João | |
| person.identifier.ciencia-id | FD19-02DC-1EE2 | |
| person.identifier.orcid | 0000-0001-9483-5225 | |
| person.identifier.scopus-author-id | 7403252256 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | fa25995a-3240-452c-b5a7-a48714e6cf9e | |
| relation.isAuthorOfPublication.latestForDiscovery | fa25995a-3240-452c-b5a7-a48714e6cf9e |