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Sequential injection lab-on-valve system for the for the determination of the activity of peroxidase in vegetables

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dc.contributor.authorVidigal, Susana S. M. P.
dc.contributor.authorToth, Ildiko V.
dc.contributor.authorRangel, António O. S. S.
dc.date.accessioned2010-10-06T11:12:36Z
dc.date.available2010-10-06T11:12:36Z
dc.date.issued2010
dc.description.abstractHorseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometric determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential injection lab-on-valve format. The developed system is based on the reaction between hydrogen peroxide (H2O2) and 2,2-azinobis(3-ethylbenzothiazoline-6)sulfonic acid (ABTS) catalyzed by the enzyme (HRP). The method presented a sample consumption of 15 μL per assay and a consumption of ABTS and H2O2 of 24 μg and 12 μg per assay, respectively. It was also possible to monitor online the thermal inactivation of peroxidase at different temperature ranges.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Journal of Agricultural and Food Chemistry". ISSN 1520-5118. 58: 4 (2010) 2071–2075por
dc.identifier.doi10.1021/jf9035113
dc.identifier.eissn1520-5118
dc.identifier.issn0021-8561
dc.identifier.urihttp://hdl.handle.net/10400.14/2662
dc.identifier.wosWOS:000274530100003
dc.language.isoengpor
dc.publisherAmerican Chemical Societypor
dc.subjectSequential injection lab-on-valvepor
dc.subjectSpectrophotometrypor
dc.subjectHorseradish peroxidasepor
dc.subjectVegetablespor
dc.subjectThermal inactivationpor
dc.titleSequential injection lab-on-valve system for the for the determination of the activity of peroxidase in vegetablespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2075
oaire.citation.issue4
oaire.citation.startPage2071
oaire.citation.titleJournal of Agricultural and Food Chemistry
oaire.citation.volume58
person.familyNameVidigal
person.familyNameTóth
person.familyNameSantos Silva Rangel
person.givenNameSusana
person.givenNameIldikó
person.givenNameAntónio Osmaro
person.identifier.ciencia-id2919-B77C-11C0
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0002-0213-063X
person.identifier.orcid0000-0002-7342-1063
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridM-4661-2013
person.identifier.ridG-1733-2010
person.identifier.scopus-author-id11739488200
person.identifier.scopus-author-id35512332800
person.identifier.scopus-author-id7004827387
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication59453cc2-ee50-4a1d-bbfc-924ca6417004
relation.isAuthorOfPublication7889563f-f3fc-4057-a97c-b51425b4dc46
relation.isAuthorOfPublication467be022-fd9c-4549-aa48-48ebcc1098eb
relation.isAuthorOfPublication.latestForDiscovery7889563f-f3fc-4057-a97c-b51425b4dc46

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