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Molecular techniques and target selection for the identification of Candida spp. in oral samples

dc.contributor.authorMagalhães, Joana
dc.contributor.authorCorreia, Maria José
dc.contributor.authorSilva, Raquel M.
dc.contributor.authorEsteves, Ana Cristina
dc.contributor.authorAlves, Artur
dc.contributor.authorDuarte, Ana Sofia
dc.date.accessioned2022-09-28T12:36:14Z
dc.date.available2022-09-28T12:36:14Z
dc.date.issued2022-09-14
dc.description.abstractCandida species are the causative agent of oral candidiasis, with medical devices being platforms for yeast anchoring and tissue colonization. Identifying the infectious agent involved in candidiasis avoids an empirical prescription of antifungal drugs. The application of high-throughput technologies to the diagnosis of yeast pathogens has clear advantages in sensitivity, accuracy, and speed. Yet, conventional techniques for the identification of Candida isolates are still routine in clinical and research settings. Molecular approaches are the focus of intensive research, but conversion into clinic settings requires overcoming important challenges. Several molecular approaches can accurately identify Candida spp.: Polymerase Chain Reaction, Microarray, High-Resolution Melting Analysis, Multi-Locus Sequence Typing, Restriction Fragment Length Polymorphism, Loop-mediated Isothermal Amplification, Matrix Assisted Laser Desorption Ionization-mass spectrometry, and Next Generation Sequencing. This review examines the advantages and disadvantages of the current molecular methods used for Candida spp. Identification, with a special focus on oral candidiasis. Discussion regarding their application for the diagnosis of oral infections aims to identify the most rapid, affordable, accurate, and easy-to-perform molecular techniques to be used as a point-of-care testing method. Special emphasis is given to the difficulties that health care professionals need to overcome to provide an accurate diagnosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/app12189204pt_PT
dc.identifier.eid85138684762
dc.identifier.issn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.14/39028
dc.identifier.wos000856212300001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDiagnosispt_PT
dc.subjectInfectionpt_PT
dc.subjectOral candidiasispt_PT
dc.subjectOral healthpt_PT
dc.subjectSpecies identificationpt_PT
dc.titleMolecular techniques and target selection for the identification of Candida spp. in oral samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue18pt_PT
oaire.citation.titleApplied Sciences (Switzerland)pt_PT
oaire.citation.volume12pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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