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Abstract(s)
Two bacterial strains, G30T and A1PC16, isolated respectively from raw milk and raw wastewater, were characterized using a polyphasic approach. Chemotaxonomic characterization supported
the inclusion of these strains in the genus Acinetobacter, with Q-8 and Q-9 as the major
respiratory quinones, genomic DNA G+C contents within the range observed for this genus(38–47 mol%) and C16 : 0, C18 : 1v9c and C16 : 1v7c/iso-C15 : 0 2-OH as the predominant fatty
acids. The observation of 16S rRNA gene sequence similarity lower than 97% with other
Acinetobacter species with validly published names led to the hypothesis that these isolates could
represent a novel species. This hypothesis was supported by comparative analysis of partial
sequences of the genes rpoB and gyrB, which showed that strains G30T and A1PC16 did not
cluster with any species with validly published names, forming a distinct lineage. DNA–DNA
hybridizations confirmed that the two strains were members of the same species, which could be
distinguished from their congeners by several phenotypic characteristics. On the basis of these
arguments, it is proposed that strains G30T and A1PC16 represent a novel species, for which the
name Acinetobacter rudis sp. nov. is proposed. The type strain is strain G30T (5LMG 26107T
5CCUG 57889T 5DSM 24031T 5CECT 7818T).
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Citation
VAZ-MOREIRA, Ana ...[et al.] - Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater. International Journal of Systematic and Evolutionary Microbiology. ISSN:1466-5034. Vol. 61, n.º12 (2011), p. 2837-2843
Publisher
Microbiology Society