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Hydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process

2020Journal article Restricted access
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dc.contributor.authorVeiga, Anabela
dc.contributor.authorCastro, Filipa
dc.contributor.authorCunha-Reis, Cassilda
dc.contributor.authorSousa, Aureliana
dc.contributor.authorOliveira, Ana
dc.contributor.authorRocha, Fernando
dc.date.accessioned2020-01-07T19:11:32Z
dc.date.available2020-01-07T19:11:32Z
dc.date.issued2020
dc.description.abstractSynthesis of hydroxyapatite (HAp) and sericin (SS) nanocomposites was carried out by a simple precipitation method performed in batch in a stirred tank reactor (ST). The reaction was achieved by mixing a solution of calcium chloride dihydrate, in which SS was dissolved, with a solution of disodium hydrogen phosphate at 37 °C. Three experimental conditions were studied by varying the concentration of SS: HAp, HAp/SS1 (0.01 g/L of SS) and HAp/SS2 (1 g/L of SS). The chemical and physical properties of the resulting HAp/SS nanocomposites were studied using several techniques (Atomic Absorption Spectrometry, Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Selected area diffraction (SAED) and Thermogravimetric analysis (TGA)). pH profile was also monitored over time for each experimental condition. The results revealed that nano single-phased HAp was formed with both rod and plate-like shape. Additionally, the particles have low crystallinity, characteristic similar to biological HAp. Regarding the influence of SS, one observed that with increasing SS concentration there is an increase in the mean particle size and the number of plate-like particles, as well as an increase in the aggregation degree and a decrease of the crystallinity. Further, the composites obtained have an inorganic/organic composition comparable to bone. Finally, in vitro cytotoxicity showed that the synthetized nanoparticles are non-toxic and cell viability is higher for HAp and HAp/SS samples when compared to a commercially available HAp. The produced materials can thus be considered suitable candidates for bone related applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVeiga, A., Castro, F., Reis, C. C., Sousa, A., Oliveira, A. L., Rocha, F. (2020). Hydroxyapatite/sericin composites: a simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process. Materials Science and Engineering: C, 108, 110400pt_PT
dc.identifier.doi10.1016/j.msec.2019.110400pt_PT
dc.identifier.eid85075198665
dc.identifier.issn0928-4931
dc.identifier.pmid31923995
dc.identifier.urihttp://hdl.handle.net/10400.14/29109
dc.identifier.wos000510956400048
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPTDC/QEQPRS/3787/2014pt_PT
dc.relationLaboratory for Process Engineering, Environment, Biotechnology and Energy
dc.relationCentre for Biotechnology and Fine Chemistry
dc.subjectHydroxyapatite (HAp)pt_PT
dc.subjectSericin (SS)pt_PT
dc.subjectNanocompositespt_PT
dc.subjectPrecipitationpt_PT
dc.subjectStirred tank (ST)pt_PT
dc.titleHydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization processpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLaboratory for Process Engineering, Environment, Biotechnology and Energy
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FEQU%2F00511%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F00511%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.citation.titleMaterials Science and Engineering: Cpt_PT
oaire.citation.volume108pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream5876
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCunha Reis
person.givenNameCassilda
person.identifier.ciencia-idE718-0A09-A959
person.identifier.orcid0000-0001-6886-3900
person.identifier.scopus-author-id20336729000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3000922a-5ab0-4d36-8fd3-e88bfca427f1
relation.isAuthorOfPublication.latestForDiscovery3000922a-5ab0-4d36-8fd3-e88bfca427f1
relation.isProjectOfPublicationeb22d744-1781-43b3-8f10-5b71f3f0b30b
relation.isProjectOfPublication75415342-6720-45a7-bc80-eec5195ad60e
relation.isProjectOfPublication1f76a7db-2520-482f-b56b-7e01ea36dd1f
relation.isProjectOfPublication.latestForDiscovery75415342-6720-45a7-bc80-eec5195ad60e

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