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Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability

2001Journal article Open access
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dc.contributor.authorMendes-Pinto, M.M.
dc.contributor.authorRaposo, M.F.J.
dc.contributor.authorBowen, J.
dc.contributor.authorYoung, A.J.
dc.contributor.authorMorais, R.
dc.date.accessioned2011-10-22T11:47:55Z
dc.date.available2011-10-22T11:47:55Z
dc.date.issued2001
dc.description.abstractAlthough Haematococcus pluvialis is one of the most importantnatural sources of the carotenoid astaxanthin as a pigmentor for theaquaculture industry, the thick sporopollenin cell wall in the cysts hindersastaxanthin extraction and its subsequent bio-availability to fish. A rangeof physical and chemical processes were tested to promote the disruptionof the encysted cells. The efficacy of these processes was evaluated interms of astaxanthin recovery, which was assessed by determining theextent of leaching of astaxanthin into an organic solvent. The processestested were: autoclave 30 min, 121 °C, 1 atm; HCl 0.1 M, 15min and 30 min; NaOH 0.1 M, 15 min and 30 min; enzymatictreatment with a mixture of 0.1% protease K and 0.5% driselase in aphosphate buffer, pH 5.8, 30 °C, for one hour; spray drying, inlet180 °C, outlet 115 °C; and mechanical disruption, with acell homogeniser developed for this purpose. The mechanical(homogenisation) and autoclave treatments were the most effective in termsof extraction and availability.por
dc.identifier.citationMENDES-PINTO, M.M...[et al.] - Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability. Journal of Applied Phycology. ISSN 1573-5176. Vol. 13, n.º 1 (2001), p. 19–24por
dc.identifier.doi10.1023/A:1008183429747
dc.identifier.urihttp://hdl.handle.net/10400.14/6801
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringel Verlagpor
dc.subjectAstaxanthinpor
dc.subjectBio-availabilitypor
dc.subjectHaematococcuspor
dc.subjectMicroalgaepor
dc.subjectProcessingpor
dc.titleEvaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availabilitypor
dc.typejournal article
dspace.entity.typePublication
person.familyNamede Jesus Raposo
person.familyNameMorais
person.givenNameMaria Filomena
person.givenNameRui M. S. C.
person.identifier143926
person.identifier.ciencia-id4F1A-88A7-FB5C
person.identifier.ciencia-id541B-04AE-0686
person.identifier.orcid0000-0002-2411-0410
person.identifier.orcid0000-0001-5138-6941
person.identifier.ridM-5065-2013
person.identifier.scopus-author-id7005684061
person.identifier.scopus-author-id7005146997
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3a13dab3-c68c-4db4-a3a1-6ba8507f92b5
relation.isAuthorOfPublicationfa2b729c-8159-4a98-9bfe-0fa1e98aa9fb
relation.isAuthorOfPublication.latestForDiscoveryfa2b729c-8159-4a98-9bfe-0fa1e98aa9fb

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