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Biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11

2022-11-18Journal article Open access
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dc.contributor.authorBoufercha, Oumeima
dc.contributor.authorMonforte, Ana R.
dc.contributor.authorBoudemagh, Allaoueddine
dc.contributor.authorFerreira, António C.
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorMoreira, Irina S.
dc.date.accessioned2022-12-06T12:58:08Z
dc.date.available2022-12-06T12:58:08Z
dc.date.issued2022-11-18
dc.description.abstractThiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by Labrys portucalensis F11. After 30 days of incubation in mineral salt medium, L. portucalensis F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L−1) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L−1); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using Aliivibrio fischeri and by assessing the synthesis of an inducible β-galactosidase by an E. coli mutant (Toxi-Chromo test). L. portucalensis F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms232214326pt_PT
dc.identifier.eid85142787446
dc.identifier.issn1661-6596
dc.identifier.pmcPMC9694413
dc.identifier.pmid36430799
dc.identifier.urihttp://hdl.handle.net/10400.14/39484
dc.identifier.wos000887299900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLabrys portucalensis F11pt_PT
dc.subjectBiodegradationpt_PT
dc.subjectMetabolitespt_PT
dc.subjectThiamethoxampt_PT
dc.subjectToxicitypt_PT
dc.titleBiodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by Labrys portucalensis F11pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue22pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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