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Toxicity of recombinant necrosis and ethylene-inducing proteins (NLPs) from Neofusicoccum parvum

2020-04-01Journal article Open access
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dc.contributor.authorPour, Forough Nazar
dc.contributor.authorCobos, Rebeca
dc.contributor.authorCoque, Juan José Rubio
dc.contributor.authorSerôdio, João
dc.contributor.authorAlves, Artur
dc.contributor.authorFélix, Carina
dc.contributor.authorFerreira, Vanessa
dc.contributor.authorEsteves, Ana Cristina
dc.contributor.authorDuarte, Ana Sofia
dc.date.accessioned2021-03-19T13:13:45Z
dc.date.available2021-03-19T13:13:45Z
dc.date.issued2020-04-01
dc.description.abstractNeofusicoccum parvum is a fungal pathogen associated with a wide range of plant hosts. Despite being widely studied, the molecular mechanism of infection of N. parvum is still far from being understood. Analysis of N. parvum genome lead to the identification of six putative genes encoding necrosis and ethylene-inducing proteins (NLPs). The sequence of NLPs genes (NprvNep 1-6) were analyzed and four of the six NLP genes were successfully cloned, expressed in E. coli and purified by affinity chromatography. Pure recombinant proteins were characterized according to their phytotoxic and cytotoxic effects to tomato leaves and to mammalian Vero cells, respectively. These assays revealed that all NprvNeps tested are cytotoxic to Vero cells and also induce cell death in tomato leaves. NprvNep2 was the most toxic to Vero cells, followed by NprvNep1 and 3. NprvNep4 induced weaker, but, nevertheless, still significant toxic effects to Vero cells. A similar trend of toxicity was observed in tomato leaves: the most toxic was NprvNep 2 and the least toxic NprvNep 4. This study describes for the first time an overview of the NLP gene family of N. parvum and provides additional insights into its pathogenicity mechanism.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/toxins12040235pt_PT
dc.identifier.eid85083251939
dc.identifier.issn2072-6651
dc.identifier.pmcPMC7232490
dc.identifier.pmid32272814
dc.identifier.urihttp://hdl.handle.net/10400.14/32284
dc.identifier.wos000533393900055
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBotryosphaeria diebackpt_PT
dc.subjectBotryosphaeriaceaept_PT
dc.subjectCytotoxicitypt_PT
dc.subjectNecrosis and ethylene-inducing proteins (NLPs)pt_PT
dc.subjectPhytotoxicitypt_PT
dc.titleToxicity of recombinant necrosis and ethylene-inducing proteins (NLPs) from Neofusicoccum parvumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.titleToxinspt_PT
oaire.citation.volume12pt_PT
person.familyNameCobos
person.familyNameRubio Coque
person.familyNameSerôdio
person.familyNameAlves
person.familyNameFélix
person.familyNameEsteves
person.familyNameDuarte
person.givenNameRebeca
person.givenNameJuan José
person.givenNameJoão
person.givenNameArtur
person.givenNameCarina
person.givenNameAna Cristina
person.givenNameAna Sofia
person.identifier630341
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person.identifier.ridB-8201-2008
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person.identifier.scopus-author-id24479329400
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person.identifier.scopus-author-id56379803400
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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