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Atomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureus

2008Journal article Restricted access
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dc.contributor.authorEaton, Peter
dc.contributor.authorFernandes, João C.
dc.contributor.authorPereira, Eulália
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2010-10-08T17:12:26Z
dc.date.available2010-10-08T17:12:26Z
dc.date.issued2008
dc.description.abstractChitosan has been reported to be a non-toxic, biodegradable antibacterial agent. The aim of this work was to elucidate the relationship between the molecular weight of chitosan and its antimicrobial activity upon two model microorganisms, one Gram-positive (Staphylococcus aureus) and one Gramnegative (Escherichia coli). Atomic force microscopy (AFM) imaging was used to obtain high-resolution images of the effect of chitosans on the bacterial morphology. The AFM measurements were correlated with viable cell numbers, which show that the two species reacted differently to the high- and lowmolecular-weight chitosan derivatives. The images obtained revealed not only the antibacterial effects, but also the response strategies used by the bacteria; cell wall collapse and morphological changes reflected cell death, whereas clustering of bacteria appeared to be associated with cell survival. In addition, nanoindentation experiments with the AFM revealed mechanical changes in the bacterial cell wall induced by the treatment. The nanoindentation results suggested that despite little modification observed in the Gram-positive bacteria in morphological studies, cell wall damage had indeed occurred, since cell wall stiffness was reduced after chitooligosaccharide treatment.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Ultramicroscopy". ISSN 0304-3991. 108: 10 (2008) 1128– 1134por
dc.identifier.doi10.1016/j.ultramic.2008.04.015
dc.identifier.urihttp://hdl.handle.net/10400.14/2733
dc.language.isoengpor
dc.publisherElsevierpor
dc.subjectAtomic force microscopypor
dc.subjectChitosanpor
dc.subjectAntimicrobialpor
dc.subjectChitooligosaccharidespor
dc.subjectCell wallpor
dc.subjectNanoindentationpor
dc.titleAtomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureuspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameFernandes
person.familyNamePintado
person.familyNameMalcata
person.givenNameJoão
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-id881D-7FFD-DD7D
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-1556-1698
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-3126-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id35274726800
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4f081a71-52de-47ab-80f1-45a546c3ebab
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relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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