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Bioengineered 3D microvessels reveal novel determinants of Trypanosoma congolense sequestration

2024-09-25Preprint Acesso aberto
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dc.contributor.authorPorqueddu, Teresa
dc.contributor.authorNiz, Mariana De
dc.contributor.authorCasas-Sánchez, Aitor
dc.contributor.authorIntroini, Viola
dc.contributor.authorZorrinho-Almeida, Maria
dc.contributor.authorSender, Silvia Sanz
dc.contributor.authorCarrasqueira, Diana
dc.contributor.authorFigueiredo, Luisa
dc.contributor.authorBernabeu, Maria
dc.contributor.authorPereira, Sara Silva
dc.date.accessioned2026-07-10T15:28:35Z
dc.date.available2026-07-10T15:28:35Z
dc.date.issued2024-09-25
dc.description.abstractIn the mammalian host, Trypanosoma congolense cytoadheres to the vascular endothelium in a process known as sequestration. Although sequestration influences clinical outcome, disease severity and organ pathology, its determinants and mediators remain unknown. Challenges such as the variability of animal models, the only-recently developed tools to genetically manipulate the parasite, and the lack of physiologically relevant in vitro models have hindered progress. Here, we engineered brain and cardiac 3D bovine endothelial microvessel models that mimic the bovine brain microvasculature and the bovine aorta, respectively. By perfusing these models with two T. congolense strains, we simulated physiologically relevant conditions and investigated the roles of flow for parasite sequestration and tropism for different endothelial beds. We discovered that sequestration is dependent on cyclic AMP signalling, closely linked to parasite proliferation, but not associated with parasite transmission to the tsetse fly vector. Finally, by comparing the expression profiles of sequestered and non-sequestered parasites collected from a rodent model, we showed gene expression changes in sequestered parasites, including of the surface variant antigens. This work presents a physiologically-relevant platform to study trypanosome interactions with the vasculature and provides a deeper understanding of the molecular and biophysical mechanisms underlying T. congolense sequestration.eng
dc.identifier.doi10.21203/rs.3.rs-5104603/v1
dc.identifier.otherf46b1c15-ecf3-46cb-8430-24ed0a74b527
dc.identifier.urihttp://hdl.handle.net/10400.14/58661
dc.language.isoeng
dc.publisherSpringer Science and Business Media, LLC
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSequestrationeng
dc.subjectTrypanosoma congolenseeng
dc.subjectAnimal African trypanosomiasiseng
dc.subjectVascular bioengineeringeng
dc.titleBioengineered 3D microvessels reveal novel determinants of Trypanosoma congolense sequestration
dc.typepreprint
dspace.entity.typePublication
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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