Repository logo
 
Publication

Survival of listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses

2017Journal article Restricted access
Simple item page
dc.contributor.authorKomora, Norton
dc.contributor.authorBruschi, Carolina
dc.contributor.authorMagalhães, Rui
dc.contributor.authorFerreira, Vânia
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2017-12-18T19:40:28Z
dc.date.available2017-12-18T19:40:28Z
dc.date.issued2017
dc.description.abstractThe ongoing rise of antibiotic resistant microbial pathogens has become one of the major public health threats worldwide. Despite all the effort and actions taken so far, a proliferation of antibiotic resistant (AR) and multi-antibiotic resistant (MAR) strains is still observed, including in foodborne pathogens. This trend has been also noted recently for isolates of Listeria monocytogenes, a species that, although remaining largely sensitive to clinically relevant antimicrobials, has been reported to develop increased tolerance to antibiotics, particularly in isolates recovered from the food-chain. In this study we compared the ability of MAR (n=8), AR (n=18) and antibiotic susceptible (AS, n=11) L. monocytogenes strains from food and clinical origin to survive to different environmental stress conditions, including temperature (58°C), acidic stress (1% v/v lactic acid, pH3.5), and osmotic stress (37% w/v NaCl). The presence of antibiotic active efflux among MAR and AR strains, and its role on L. monocytogenes tolerance to different antimicrobial compounds was also investigated, namely; hydrogen peroxide; organic acids (acetic, citric and lactic); nisin; benzalkonium chloride (BC); and, sodium nitrite. While no significant differences were observed in the survival of the 37 strains exposed to high temperature (58°C), overall the mean logarithmic reduction of clinical strains was statistically lower after acid and salt exposure than that observed for strains of food origin; but both food and clinical strains resistant to two or three antibiotics were significantly less susceptible to acid (lactic acid 1% v/v) and osmotic stresses (37% w/v NaCl) when compared to AS strains. Using the EtBr-agar Cartwheel method, it was possible to detect efflux pumps in three of the 26 MAR and AR isolates, including one control strain; the active efflux in theses isolates was proven to be associated with fluoroquinolone resistance, and possible extrusion of BC and hydrogen peroxide. The mechanisms responsible for the possible correlation between resistance to antibiotics and to acid or salt stress in L. monocytogenes have yet to be understood.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationKomora, N., Bruschi, C., Magalhaes, R., Ferreira, V., & Teixeira, P. (2017). Survival of listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses. International Journal of Food Microbiology, 245, 79-87pt_PT
dc.identifier.doi10.1016/j.ijfoodmicro.2017.01.013pt_PT
dc.identifier.eid85010867659
dc.identifier.eissn1879-3460
dc.identifier.issn0168-1605
dc.identifier.pmid28157581
dc.identifier.urihttp://hdl.handle.net/10400.14/23729
dc.identifier.wos000395599700011
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-07-0124-FEDER-000052pt_PT
dc.relationCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
dc.subjectListeria monocytogenespt_PT
dc.subjectAntibioticpt_PT
dc.subjectResistancept_PT
dc.subjectEfflux pumppt_PT
dc.subjectStresspt_PT
dc.subjectEtBr-agar cartwheel methodpt_PT
dc.titleSurvival of listeria monocytogenes with different antibiotic resistance patterns to food-associated stressespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71704%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72617%2F2010/PT
oaire.citation.endPage87pt_PT
oaire.citation.startPage79pt_PT
oaire.citation.titleInternational Journal of Food Microbiologypt_PT
oaire.citation.volume245pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameKomora
person.familyNameMagalhães
person.familyNameBorges Ferreira
person.familyNameTeixeira
person.givenNameNorton
person.givenNameRui
person.givenNameVânia
person.givenNamePaula
person.identifierK-1359-2014
person.identifier.ciencia-idD114-BCFA-5EEB
person.identifier.ciencia-id4C1A-BDD4-D716
person.identifier.orcid0000-0002-1118-3629
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0001-7093-9193
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridK-1359-2014
person.identifier.ridK-1343-2014
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id57039111500
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id8317338900
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione201a9dd-e99a-4726-9913-66e7664d9071
relation.isAuthorOfPublicationb0b10107-23c3-4029-9373-3865df284308
relation.isAuthorOfPublicationb8867092-8efe-4663-99d3-7cb6d1a7d89e
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryb0b10107-23c3-4029-9373-3865df284308
relation.isProjectOfPublication3fe5970e-de39-42f7-a29d-18521b591a09
relation.isProjectOfPublicationf7e2530f-03f2-475c-a6a9-f0b7d97f651a
relation.isProjectOfPublication1d38b489-b68f-4e75-a8ba-b3a6d1db48b9
relation.isProjectOfPublicationfb80a455-1971-4d70-a7a6-9e1bad0e5933
relation.isProjectOfPublication.latestForDiscovery1d38b489-b68f-4e75-a8ba-b3a6d1db48b9

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
19779869.pdf
Size:
771.04 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals