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Microbial degradation of pharmaceuticals followed by a simple HPLC-DAD method

2012Journal article Restricted access
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dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorGoncalves, Virgínia M.F.
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorCarvalho, Maria F.
dc.contributor.authorCastro, Paula M.L.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2013-05-02T13:56:38Z
dc.date.available2013-05-02T13:56:38Z
dc.date.issued2012
dc.description.abstractThe biodegradation of five pharmaceutical ingredients (PIs) of different therapeutic classes, namely antibiotics (trimethoprim, sulfametoxazole and ciprofloxacin), anti-inflammatory (diclofenac) and anti-epileptic (carbamazepine), by two distinct microbial consortia, was investigated. For the monitoring of biodegradation assays, a simple HPLC-DAD (High Performance Liquid Chromatography – Diode Array Detector) method was developed and validated. The separation of the target pharmaceuticals was performed using an environmental friendly mobile phase in a gradient mode of 0.1% triethylamine (TEA) in water acidified at pH 2.23 with trifluoroacetic acid (TFAA) and ethanol as organic solvent. The method revealed to be selective, linear and precise in the range of 1.0 to 30.0 μg/mL for all PIs. Biodegradation assays were performed using activated sludge and a bacterial consortium (able to degrade fluoroaromatic compounds) supplemented with the target PIs at a final concentration of 25 μg/mL. The results revealed that activated sludge removed the target compounds more efficiently than the bacterial consortium.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRIBEIRO, Ana R… [et al.] - Microbial degradation of pharmaceuticals followed by a simple HPLC-DAD method. Journal of Environmental Science and Health: Part A. ISSN 1532-4117. Vol. 47, n.º 13 (2012), p. 2151-2158por
dc.identifier.doi10.1080/10934529.2012.696422
dc.identifier.eissn1532-4117
dc.identifier.issn1093-4529
dc.identifier.urihttp://hdl.handle.net/10400.14/10397
dc.identifier.wosWOS:000307647900022
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherTaylor & Francispor
dc.relation09-GCQF-CICS-09
dc.relationPTDC/EBBEBI/111699/2009
dc.relationBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
dc.subjectHPLC-DADpor
dc.subjectBiodegradationpor
dc.subjectPersistent pharmaceuticalspor
dc.subjectActivated sludgepor
dc.subjectCarbamazepinepor
dc.subjectFluoroaromatic compoundspor
dc.titleMicrobial degradation of pharmaceuticals followed by a simple HPLC-DAD methodpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64999%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44670%2F2008/PT
oaire.citation.endPage2158
oaire.citation.issue13
oaire.citation.issueJournal of Environmental Science and Health: Part A
oaire.citation.startPage2151
oaire.citation.volume47
oaire.fundingStreamSFRH
person.familyNameRibeiro
person.familyNameFerreira Gonçalves
person.familyNameMaia
person.familyNameCarvalho
person.familyNameCastro
person.familyNameTiritan
person.givenNameAna Rita Lado
person.givenNameVirgínia Maria
person.givenNameAlexandra S.
person.givenNameM. Fátima
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier903786
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id421D-DC6E-F6E3
person.identifier.ciencia-idDB12-D51F-C2D7
person.identifier.ciencia-id6A15-F0A3-AEF2
person.identifier.ciencia-id6913-1464-155B
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4445-5379
person.identifier.orcid0000-0002-9151-516X
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0002-7181-0540
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id57207918943
person.identifier.scopus-author-id23097687200
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id14038677200
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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