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Characterization of a lactiplantibacillus plantarum r23 isolated from arugula by whole-genome sequencing and its bacteriocin production ability

2021-06-01Journal article Open access
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dc.contributor.authorBarbosa, Joana
dc.contributor.authorAlbano, Helena
dc.contributor.authorSilva, Beatriz
dc.contributor.authorAlmeida, Maria Helena
dc.contributor.authorNogueira, Teresa
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2021-06-06T19:24:39Z
dc.date.available2021-06-06T19:24:39Z
dc.date.issued2021-06-01
dc.description.abstractLactiplantibacillus plantarum is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers Lpb. plantarum are also promising natural food preservatives. This study aimed to characterize Lpb. plantarum R23 and its bacteriocins (R23 bacteriocins). The genome sequence of Lpb. plantarum R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of Lpb. plantarum R23-produced bacteriocin against two Listeria monocytogenes strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pedi-ocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (<6.5 kDa) R23 bacteriocins were stable at different pH values (ranging from 2 to 8), temperatures (between 4 and 100 °C), detergents (all, except Triton X-100 and Triton X-114 at 0.01 g/mL), and enzymes (catalase and α-amylase), did not adsorb to the producer cells, had a bacteriostatic mode of action and their maximum activity (AU/mL = 12,800) against two L. monocytogenes strains occurred between 15 and 21 h of Lpb. plantarum R23 growth. Lactiplantibacillus plantarum R23 showed to be a promising bio-preservative culture because, besides being safe, it produces a stable bacteriocin or bacteriocins (har-bors genes encoding for the production of four) inhibiting pathogens as L. monocytogenes. Further studies in different food matrices are required to confirm this hypothesis and its suitability as a future starter culture.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijerph18115515pt_PT
dc.identifier.eid85106240153
dc.identifier.issn1661-7827
dc.identifier.pmcPMC8196627
dc.identifier.pmid34063896
dc.identifier.urihttp://hdl.handle.net/10400.14/33440
dc.identifier.wos000659930400001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBio-preservationpt_PT
dc.subjectCoagulin Apt_PT
dc.subjectFood safetypt_PT
dc.subjectPediocinpt_PT
dc.subjectPlantaricinpt_PT
dc.subjectStarter culturept_PT
dc.titleCharacterization of a lactiplantibacillus plantarum r23 isolated from arugula by whole-genome sequencing and its bacteriocin production abilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume18pt_PT
person.familyNameBarbosa
person.familyNameAlbano
person.familyNameNogueira
person.familyNameTeixeira
person.givenNameJoana
person.givenNameHelena
person.givenNameTeresa
person.givenNamePaula
person.identifier888247
person.identifier.ciencia-id9516-589F-8B0D
person.identifier.ciencia-id201A-0D1F-C0FC
person.identifier.ciencia-idF211-4E18-2C69
person.identifier.orcid0000-0001-5879-9458
person.identifier.orcid0000-0002-2798-1924
person.identifier.orcid0000-0002-0059-5177
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridK-3400-2014
person.identifier.ridG-2273-2010
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id13608752600
person.identifier.scopus-author-id6602934854
person.identifier.scopus-author-id7004351448
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd48923b4-519c-419b-b105-6a8ba5ed02ac
relation.isAuthorOfPublication2f53d2f8-516d-48fd-a5d9-671404e1a456
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b

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