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Partial identification of water-soluble peptides released at early stages of proteolysis in sterilized ovine cheese-like systems: Influence of type of coagulant and starter

2005Journal article Open access
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dc.contributor.authorSilva, S. V.
dc.contributor.authorMalcata, F. X.
dc.date.accessioned2011-10-21T16:26:32Z
dc.date.available2011-10-21T16:26:32Z
dc.date.issued2005
dc.description.abstractCheese-like systems were manufactured from sterilized ovine milk, using crude aqueous extracts of Cynara cardunculus or cardosin A isolated therefrom as clotting agent. The effect of adding a commercial starter culture was also assessed. The impact of the type of coagulant used during the initial 24 h of proteolysis was evaluated via separation of peptides in the watersoluble extracts by reverse-phase HPLC, followed by partial sequencing via Edman degradation. Cardosin A accounted for most events of primary proteolysis. The major cleavage sites were Phe105-Met106 in κ-casein, and Leu127-Thr128, Ser142-Trp143, Leu165-Ser166, and Leu190-Tyr191 in β-casein. The starter culture did not play an active role during the initial stages of ripening.por
dc.identifier.citationSILVA, S. V.; MALCATA, F.X. - Partial identification of water-soluble peptides released at early stages of proteolysis in sterilized ovine cheese-like systems: Influence of type of coagulant and starter. Journal of Dairy Science. ISSN 0022-0302. 88:6 (2005) 1947-1954por
dc.identifier.urihttp://hdl.handle.net/10400.14/6738
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Dairy Science Associationpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0022030205728708por
dc.subjectCasein hydrolysispor
dc.subjectCynara cardunculuspor
dc.subjectRennetpor
dc.subjectRP-HPLCpor
dc.titlePartial identification of water-soluble peptides released at early stages of proteolysis in sterilized ovine cheese-like systems: Influence of type of coagulant and starterpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMalcata
person.givenNameFrancisco
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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