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Abstract(s)
Para a realização deste estudo, procedeu-se à análise comparativa entre a diferença de mesófilos a 22º C em UFC/ml, provenientes de três laboratórios - o Laboratório de Pedras Salgadas, o Laboratório Central de Leça do Balio e Laboratório Externo Pimenta do Vale. De forma similar, se procedeu à comparação de UFC/ml de mesófilos a 22º C com meio PCA em banho de arrefecimento a 45º C a 52º C. Validado por ser mais rápido e eficiente, o meio cromogénico Chromagar Pseudomonas, meio de confirmação de Pseudomonas aeruginosa, contém enzimas específicas que expressa as colónias com a cor característica da bactéria. No Laboratório de Pedras Salgadas implementou-se um método de confirmação de Staphylococcus totais e Staphlococcus aureus, seguindo/obdecendo/cumprindo a norma 4343. Este estágio estabeleceu, como principais objetivos, realizar a identificação, validação e aperfeiçoamento de alguns métodos de controlo de qualidade no centro de produção de Pedras Salgadas, comparando-os com métodos do Laboratório Externo Pimenta do Vale e a Central de Leça do Balio, de modo a diminuir discrepâncias existentes na comparação de resultados.
For this study, a comparative analysis between the difference of mesophiles at 22º C in CFU / ml from three laboratories was performed - the Pedras Salgadas Laboratory, the Central Laboratory of Leça do Balio and the External Laboratory Pimenta do Vale. Similarly, a CFU / ml comparison of mesophiles at 22 ° C with PCA medium in a cooling bath at 45 ° C to 52 ° C was performed. Validated for being faster and more efficient, Chromagar Pseudomonas of chromogenic means, means of confirming Pseudomonas aeruginosa, contains specific enzymes that express the colonies with the characteristic color of the bacteria. At the Pedras Salgadas Laboratory a method of confirming total Staphylococcus and Staphlococcus aureus was implemented, following / obeying / complying the standard 4343 norm. This work established, as its main objectives, the identification, validation and improvement of some quality control methods in the Pedras Salgadas production center, comparing them with methods of the Pimenta do Vale External Laboratory and the Leça do Balio Central Laboratory , in order to to reduce existing discrepancies in comparing results.
For this study, a comparative analysis between the difference of mesophiles at 22º C in CFU / ml from three laboratories was performed - the Pedras Salgadas Laboratory, the Central Laboratory of Leça do Balio and the External Laboratory Pimenta do Vale. Similarly, a CFU / ml comparison of mesophiles at 22 ° C with PCA medium in a cooling bath at 45 ° C to 52 ° C was performed. Validated for being faster and more efficient, Chromagar Pseudomonas of chromogenic means, means of confirming Pseudomonas aeruginosa, contains specific enzymes that express the colonies with the characteristic color of the bacteria. At the Pedras Salgadas Laboratory a method of confirming total Staphylococcus and Staphlococcus aureus was implemented, following / obeying / complying the standard 4343 norm. This work established, as its main objectives, the identification, validation and improvement of some quality control methods in the Pedras Salgadas production center, comparing them with methods of the Pimenta do Vale External Laboratory and the Leça do Balio Central Laboratory , in order to to reduce existing discrepancies in comparing results.
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Keywords
Mesófilos 22º C Meios cromogénicos Pseudomonas aeruginosa Staphylococcus 22º C mesophiles Chromogenic means