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Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports

2001Journal article Open access
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dc.contributor.authorLamasa, Estela M.
dc.contributor.authorBarrosa, Rui M.
dc.contributor.authorBalcão, Victor M.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-22T16:09:06Z
dc.date.available2011-10-22T16:09:06Z
dc.date.issued2001
dc.description.abstractBlends of cardosins A and B, enzymes present in aqueous extracts of the flowers of the thistle (Cynara cardunculus L.), have for long been used as rennets by the cheesemaking industry in the Iberian Peninsula. These dimeric proteases are present in the stigmæ and stylets of said flowers, and are thought to play a role in sexual reproduction of the plant. In the present research effort, production of cardosin derivatives (starting from a crude extract), encompassing full stabilization of their dimeric structure, has been attempted via covalent, multi-subunit immobilization onto highly activated agarose-glutaraldehyde supports. Boiling such enzyme derivatives in the presence of sodium dodecyl sulfate and β-mercaptoethanol did not lead to leaching of enzyme, thus proving the effectiveness of the attachment procedure. Furthermore, derivatives prepared under optimal conditions presented ca. half the specific activity of the enzyme in soluble form, and were successfully employed at lab-scale trials to perform (selective) hydrolysis of α-lactalbumin, one of the major proteins in bovine whey.por
dc.identifier.citationLAMASA, Estela M.... [et al.] - Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports.Enzyme and Microbial Technology. ISSN 0141-0229. Vol. 28, n.º 7-8 (2001), p. 642–652por
dc.identifier.doi10.1016/S0141-0229(01)00308-8
dc.identifier.urihttp://hdl.handle.net/10400.14/6836
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversiondoi:10.1016/S0141-0229(01)00308-8por
dc.subjectEnzymepor
dc.subjectCardosinpor
dc.subjectAgarosepor
dc.subjectStructural stabilizationpor
dc.subjectDairy foodspor
dc.subjectHydrolasepor
dc.subjectAttachmentpor
dc.titleHydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supportspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBalcão
person.familyNameMalcata
person.givenNameVictor
person.givenNameFrancisco
person.identifier.ciencia-idC314-A18C-931B
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-0772-2834
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridI-2182-2012
person.identifier.scopus-author-id6603449856
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9b0860f7-99b1-4ded-a483-6435882272d0
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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