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Metagenomic analysis of an urban resistome before and after wastewater treatment

dc.contributor.authorLira, Felipe
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorTamames, Javier
dc.contributor.authorManaia, Celia M.
dc.contributor.authorMartinez, Jose Luis
dc.date.accessioned2020-07-01T11:32:43Z
dc.date.available2020-07-01T11:32:43Z
dc.date.issued2020
dc.description.abstractDetermining the effect of wastewater treatment in water resistome is a topic of interest for water quality, mainly under re-use and One-Health perspectives. The resistome, the plasmidome, and the bacterial community composition of samples from influents and treated effluents from a wastewater treatment plant located in Northern Portugal were studied using metagenomic techniques. Wastewater treatment contributed to reduce the abundance of resistance genes and of plasmid replicons, coinciding with a decline in the number of intrinsic resistance genes from Enterobacteriaceae, as well as with a reduction in the relative abundance of Firmicutes and Proteobacteria after treatment. These taxons comprise bacterial pathogens, including those belonging to the ESKAPE group, which encompasses bacteria with the highest risk of acquiring antibiotic resistance, being the most relevant hosts of resistance genes acquired through horizontal gene transfer. Our results support that wastewater treatment efficiently removes the hosts of antibiotic resistance genes and, consequently, the harboured antibiotic resistance genes. Principal component analysis indicates that the resistome and the bacterial composition clustered together in influent samples, while did not cluster in final effluent samples. Our results suggest that wastewater treatment mitigates the environmental dissemination of urban resistome, through the removal of the hosts harbouring mobile resistance genes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLira, F., Vaz-Moreira, I., Tamames, J., Manaia, C. M., Martínez, J. L. (2020). Metagenomic analysis of an urban resistome before and after wastewater treatment. Scientific Reports, 10, art. n.º 8174pt_PT
dc.identifier.doi10.1038/s41598-020-65031-ypt_PT
dc.identifier.eid85084787219
dc.identifier.issn2045-2322
dc.identifier.pmcPMC7235214
dc.identifier.pmid32424207
dc.identifier.urihttp://hdl.handle.net/10400.14/30760
dc.identifier.wos000540542100006
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationRD16/0016/0011pt_PT
dc.relationS2017/BMD-3691pt_PT
dc.relationNEW INSIGHTS INTO THE ENVIRONMENTAL ANTIBIOTIC RESISTOME
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleMetagenomic analysis of an urban resistome before and after wastewater treatmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNEW INSIGHTS INTO THE ENVIRONMENTAL ANTIBIOTIC RESISTOME
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87360%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97131%2F2013/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamSFRH
person.familyNameLira Ph.D.
person.familyNameVaz-Moreira
person.familyNameManaia
person.familyNameMartinez
person.givenNameFelipe
person.givenNameIvone
person.givenNameCélia
person.givenNameJose Luis
person.identifier602434
person.identifier19844
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0002-5953-554X
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0002-3674-1789
person.identifier.orcid0000-0001-8813-7607
person.identifier.ridA-4725-2013
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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