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Use of solid phase extraction for the sequential injection determination of alkaline phosphatase activity in dynamic water systems

2012Journal article Restricted access
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dc.contributor.authorSantos, Inês C.
dc.contributor.authorMesquita, Raquel B.R.
dc.contributor.authorBordalo, Adriano A.
dc.contributor.authorRangel, António O.S.S.
dc.date.accessioned2013-01-28T12:59:57Z
dc.date.available2013-01-28T12:59:57Z
dc.date.issued2012
dc.description.abstractIn this work, a solid phase extraction sequential injection methodology for the determination of alkaline phosphatase activity in dynamic water systems was developed. The determination of the enzymatic activity was based on the spectrophotometric detection of a coloured product, p-nitrophenol, at 405 nm. The p-nitrophenol is the product of the catalytic decomposition of p-nitrophenyl phosphate, a noncoloured substrate. Considering the low levels expected in natural waters and exploiting the fact of alkaline phosphatase being a metalloprotein, the enzyme was pre-concentrated in-line using a NTA Superflow resin charged with Zn2þ ions. The developed sequential injection method enabled a quantification range of 0.044–0.441 unit mL 1 of enzyme activity with a detection limit of 0.0082 unit mL 1 enzyme activity (1.9 mmol L 1 of pNP) and a determination rate of 17 h 1. Recovery tests confirmed the accuracy of the developed sequential injection method and it was effectively applied to different natural waters and to plant root extracts.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationSANTOS, Inês C. ...[et al.] - Use of solid phase extraction for the sequential injection determination of alkaline phosphatase activity in dynamic water systems. Talanta. ISSN 0039-9140. Vol. 98, n.º 30 ( 2012), p. 203–210por
dc.identifier.doi10.1016/j.talanta.2012.06.071
dc.identifier.eissn1873-3573
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.14/9984
dc.identifier.wosWOS:000309327600029
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationDEVELOPMENT OF AUTOMATIC MICROFLOW SYSTEMS FOR MONITORING MICROBIOGICAL, BIOCHEMICAL AND PHYSICO-CHEMICAL PARAMETERS IN BATHING WATERS
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectAlkaline phosphatase activitypor
dc.subjectIn line solid phase extractionpor
dc.subjectSequential injection analysispor
dc.subjectNTA Superflow resinpor
dc.subjectSpectrophotometrypor
dc.titleUse of solid phase extraction for the sequential injection determination of alkaline phosphatase activity in dynamic water systemspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF AUTOMATIC MICROFLOW SYSTEMS FOR MONITORING MICROBIOGICAL, BIOCHEMICAL AND PHYSICO-CHEMICAL PARAMETERS IN BATHING WATERS
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F76012%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F41859%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.citation.endPage210
oaire.citation.startPage203
oaire.citation.volume98
oaire.fundingStreamSFRH
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSantos
person.familyNameMesquita
person.familyNameA. Bordalo
person.familyNameSantos Silva Rangel
person.givenNameInês
person.givenNameRaquel
person.givenNameAdriano
person.givenNameAntónio Osmaro
person.identifierJ-4630-2013
person.identifier.ciencia-idEB1D-DEE3-0B60
person.identifier.ciencia-id8B15-A841-2083
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.orcid0000-0002-4609-7085
person.identifier.orcid0000-0001-7745-1860
person.identifier.orcid0000-0002-9637-6541
person.identifier.orcid0000-0002-6486-8947
person.identifier.ridJ-4829-2013
person.identifier.ridJ-4630-2013
person.identifier.scopus-author-id36953321400
person.identifier.scopus-author-id7005556626
person.identifier.scopus-author-id7004738492
person.identifier.scopus-author-id7004827387
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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