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Genetic characterization of fluoroquinolone resistant Escherichia coli from urban streams and municipal and hospital effluents

2015Journal article Restricted access
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dc.contributor.authorVarela, Ana Rita
dc.contributor.authorMacedo, Gonçalo N.
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2016-04-21T09:28:47Z
dc.date.available2016-04-21T09:28:47Z
dc.date.issued2015
dc.description.abstractEscherichia coli with reduced susceptibility to ciprofloxacin, isolated from urban streams, wastewater treatment plants and hospital effluent between 2004 and 2012, were compared based on multilocus sequence typing (MLST), quinolone and beta-lactam resistance determinants and plasmid replicon type. Isolates from the different types of water and isolation dates clustered together, suggesting the persistence and capacity to propagate across distinct aquatic environments. Escherichia coli with reduced susceptibility to ciprofloxacin, isolated from urban streams, wastewater treatment plants and hospital effluent between 2004 and 2012, were compared based on multilocus sequence typing (MLST), quinolone and beta-lactam resistance determinants and plasmid replicon type. Isolates from the different types of water and isolation dates clustered together, suggesting the persistence and capacity to propagate across distinct aquatic environments. The most prevalent MLST groups were ST10 complex and ST131. Almost all isolates (98%) carried mutations in the chromosomal genes gyrA and/or parC, and 10% possessed the genes qepA, aac(6')-Ib-cr and/or qnrS1. Over 80% of the isolates were resistant to three or more classes of antibiotics (MDR ≤3). The most prevalent beta-lactamase encoding gene was blaTEM, followed by blaCTX-M-15, co-existing with plasmid mediated quinolone resistance. The plasmid replicon types of the group IncF were the most prevalent and distributed by different MLST groups. The genes aac(6')-Ib-cr and/or qnrS1 could be transferred by conjugation in combination with the genes blaTEM,blaSHV-12 or blaOXA-1 and the plasmid replicon types I1-Iγ, K, HI2 and/or B/O. The potential of multidrug resistant E. coli with reduced susceptibility to ciprofloxacin, harboring mobile genetic elements and with ability to conjugate and transfer resistance genes, to spread and persist across different aquatic environments was demonstrated.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationVARELA, Ana Rita; MACEDO, Gonçalo; NUNES, Olga; MANAIA, C. M. - Genetic characterization of fluoroquinolone resistant Escherichia coli from urban streams and municipal and hospital effluents. FEMS Microbiology Ecology. ISSN 1574-6941. Vol. 91 Nº. 5 (2015), 12 p. doi: 10.1093/femsec/fiv015pt_PT
dc.identifier.doi10.1093/femsec/fiv015pt_PT
dc.identifier.eid84938978684
dc.identifier.eissn1574-6941
dc.identifier.pmid25764463
dc.identifier.urihttp://hdl.handle.net/10400.14/19872
dc.identifier.wos000355328100001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press
dc.relationDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
dc.relationTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
dc.subjectWaterpt_PT
dc.subjectQuinolone resistancept_PT
dc.subjectPMQRpt_PT
dc.subjectPlasmids replicon typept_PT
dc.subjectE. coli MLSTpt_PT
dc.titleGenetic characterization of fluoroquinolone resistant Escherichia coli from urban streams and municipal and hospital effluentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDYNAMICS OF ANTIMICROBIAL RESISTANCE BETWEEN THE HOSPITAL AND THE ENVIRONMENT
oaire.awardTitleTRACKING ANTIBIOTIC RESISTANCE FROM HOSPITAL EFFLUENTS TO THE SURROUNDING ENVIRONMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAC-AMB%2F113840%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70986%2F2010/PT
oaire.citation.issue5
oaire.citation.titleFEMS Microbiology Ecology
oaire.citation.volume91
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameVarela
person.familyNameMacedo
person.familyNameNunes
person.familyNameManaia
person.givenNameAna Rita
person.givenNameGoncalo
person.givenNameOlga
person.givenNameCélia
person.identifier1189069
person.identifier.ciencia-idA710-C653-3A1C
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0001-9691-0217
person.identifier.orcid0000-0003-3595-4018
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridI-3366-2014
person.identifier.scopus-author-id56996927000
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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