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In vitro evaluation of the biological performance of macro/ micro-porous silk fibroin and silk-nano calcium phosphate scaffolds

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dc.contributor.authorYan, L.-P.
dc.contributor.authorOliveira, J. M.
dc.contributor.authorOliveira, A. L.
dc.contributor.authorReis, R. L.
dc.date.accessioned2016-04-21T16:35:16Z
dc.date.available2016-04-21T16:35:16Z
dc.date.issued2015
dc.description.abstractThis study evaluates the biological performance of salt-leached macro/microporous silk scaffolds (S16) and silk-nano calcium phosphate scaffolds (SC16), both deriving from a 16 wt % aqueous SF solution. Enzymatic degradation results showed that the silk-based scaffolds presented desirable biostability, and the incorporation of calcium phosphate further improved the scaffolds' biostability. Human adipose tissue derived stromal cells (hASCs) were cultured onto the scaffolds in vitro. The Alamar blue assay and DNA content revealed that both scaffolds were non-cytotoxic and can support the viability and proliferation of the hASCs. Scanning electron microscopy observation demonstrated that the microporous structure was beneficial for the cell adhesion while the macroporous structure favored the cell migration and proliferation. The histological analysis displayed abundant extracellular matrix formed inside the scaffolds, leading to the significant increase of scaffolds' modulus. These results revealed that S16 and SC16 could be promising alternatives for cartilage and bone tissue engineering scaffolding applications, respectively.pt_PT
dc.description.versioninfo:eu-repo/semantics/submittedVersion
dc.identifier.citationYAN, L-P. ...[et al.] - In vitro evaluation of the biological performance of macro/ micro-porous silk fibroin and silk-nano calcium phosphate scaffolds. Journal of Biomedical Materials Research Part B: Applied Biomaterials. ISSN 1552-4981. Vol 103B N.º 4 (2015), p. 888-898pt_PT
dc.identifier.doi10.1002/jbm.b.33267
dc.identifier.eid84928028994
dc.identifier.eissn1552-4981
dc.identifier.issn1552-4973
dc.identifier.pmid25164158
dc.identifier.urihttp://hdl.handle.net/10400.14/19936
dc.identifier.wos000353336800019
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley
dc.relationIF/00423/2012
dc.relationTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
dc.relationNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
dc.relationUnlocking the research potential of 3Bs Group, University of Minho, in Nanomedicine field to strengthen its competitive position at the European level
dc.subjectSilk fibroinpt_PT
dc.subjectNano calcium phosphatept_PT
dc.subjectScaffoldpt_PT
dc.subjectCytocompatibilitypt_PT
dc.subjectBiomechanical propertypt_PT
dc.titleIn vitro evaluation of the biological performance of macro/ micro-porous silk fibroin and silk-nano calcium phosphate scaffoldspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTISSUE2TISSUE-Using Textile Technologies to Develop Innovative 3D Architectures to Be Applied in Bone Tissue Engineering
oaire.awardTitleNATURE-INSPIRED SILK/POLYAMINE-CALCIUM PHOSPHATE COMPOSITES WITH CONTROLLED SURFACE TOPOGRAPHY VIA BIO-INDUCED DEPOSITION OF MULTIFUNCTIONAL SILICON-SUBSTITUTED CALCIUM PHOSPHATE FOR BONE TISSUE ENGINEERING APPLICATIONS
oaire.awardTitleUnlocking the research potential of 3Bs Group, University of Minho, in Nanomedicine field to strengthen its competitive position at the European level
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM%2F105703%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F64717%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM-BPC%2F115977%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/316331/EU
oaire.citation.endPage898
oaire.citation.issue4
oaire.citation.startPage888
oaire.citation.titleJournal of Biomedical Materials Research Part B: Applied Biomaterials
oaire.citation.volume103
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamFP7
person.familyNameYan
person.familyNameOliveira
person.familyNameReis
person.givenNameLeping
person.givenNameJoaquim Miguel
person.givenNameRui L.
person.identifier1568951
person.identifier231614
person.identifier832165
person.identifier.ciencia-idD117-EFAE-39F8
person.identifier.ciencia-id0B16-8EF6-862A
person.identifier.ciencia-id6F11-C02E-8B6C
person.identifier.orcid0000-0002-4806-5052
person.identifier.orcid0000-0001-7052-8837
person.identifier.orcid0000-0002-4295-6129
person.identifier.ridA-8622-2013
person.identifier.ridH-8636-2012
person.identifier.ridA-8938-2008
person.identifier.scopus-author-id37032413400
person.identifier.scopus-author-id57202066972
person.identifier.scopus-author-id56861715700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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