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Population structure and history of Mycobacterium bovis European 3 clonal complex reveal transmission across ecological corridors of unrecognized importance in Portugal

dc.contributor.authorPereira, André C.
dc.contributor.authorLourenço, José
dc.contributor.authorThemudo, Gonçalo
dc.contributor.authorBotelho, Ana
dc.contributor.authorCunha, Mónica V.
dc.date.accessioned2024-07-30T15:45:20Z
dc.date.available2024-07-30T15:45:20Z
dc.date.issued2024-07-02
dc.description.abstractMycobacterium bovis causes animal tuberculosis in livestock and wildlife, with an impact on animal health and production, wildlife management, and public health. In this work, we sampled a multi-host tuberculosis community from the official hotspot risk area of Portugal over 16 years, generating the largest available data set in the country. Using phylogenetic and ecological modeling, we aimed to reconstruct the history of circulating lineages across the livestock-wildlife interface to inform intervention and the implementation of genomic surveillance within the official eradication plan. We find evidence for the co-circulation of M. bovis European 1 (Eu1), Eu2, and Eu3 clonal complexes, with Eu3 providing sufficient temporal signal for further phylogenetic investigation. The Eu3 most recent common ancestor (bovine) was dated in the 1990s, subsequently transitioning to wildlife (red deer and wild boar). Isolate clustering based on sample metadata was used to inform phylogenetic inference, unravelng frequent transmission between two clusters that represent an ecological corridor of previously unrecognized importance in Portugal. The latter was associated with transmission at the livestock-wildlife interface toward locations with higher temperature and precipitation, lower agriculture and road density, and lower host densities. This is the first analysis of M. bovis Eu3 complex in Iberia, shedding light on background ecological factors underlying long-term transmission and informing where efforts could be focused within the larger hotspot risk area of Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1128/spectrum.03829-23pt_PT
dc.identifier.eid85198034853
dc.identifier.issn2165-0497
dc.identifier.pmcPMC11218495
dc.identifier.pmid38771094
dc.identifier.urihttp://hdl.handle.net/10400.14/45960
dc.identifier.wos001260892900029
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMycobacterium bovispt_PT
dc.subjectAnimal tuberculosispt_PT
dc.subjectEcological modelingpt_PT
dc.subjectPhylodynamicspt_PT
dc.subjectTransmission dynamicspt_PT
dc.subjectWhole-genome sequencingpt_PT
dc.titlePopulation structure and history of Mycobacterium bovis European 3 clonal complex reveal transmission across ecological corridors of unrecognized importance in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.titleMicrobiology spectrumpt_PT
oaire.citation.volume12pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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