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Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains

2020Journal article Restricted access
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dc.contributor.authorAlves, Ângela
dc.contributor.authorMagalhães, Rui
dc.contributor.authorBrandão, Teresa R. S.
dc.contributor.authorPimentel, Lígia
dc.contributor.authorRodríguez-Alcalá, Luis M.
dc.contributor.authorTeixeira, Paula
dc.contributor.authorFerreira, Vânia
dc.date.accessioned2019-11-06T15:17:44Z
dc.date.available2020-10-26T01:30:10Z
dc.date.issued2020
dc.description.abstractThe objective of this work was to investigate the effect of stress conditions frequently encountered in food-associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 ºC, cold stress) and optimal (37 ºC) temperatures and in high NaCl concentrations (6% NaCl, 11 ºC; cold-osmotic stress) and tested for their ability to invade the human intestinal epithelial Caco-2 cells. Results demonstrate that the correlation between exposure to cold stress and increased invasion phenotype is strain-dependent as strains investigated exhibited different behaviours, i.e. exposure to cold stress conditions resulted in a significant increase of invasion levels in five out of the eight strains tested, when compared to growth under optimal conditions. On the other hand, when these cold-adapted cells were subsequently submitted to high salt concentrations and low temperature, their enhanced ability to invade Caco-2 was lost. Surprisingly, saturated fatty acids (SFA) and branched chain fatty acids (BCFA) decreased when L. monocytogenes were exposed to stress conditions as opposed to what has been observed in other studies, therefore highlighting that further studies will need to deepen in the understanding of the lipid metabolism of these strains. The effect of stress conditions on the survival of three selected L. monocytogenes strains through an in vitro gastrointestinal (GI) tract digestion model was further investigated. The exposure to cold-osmotic stress increased the survival of one strain through the GI tract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves, Â., Magalhães, R., Brandão, T. R. S., Pimentel, L., Rodríguez-Alcalá, L. M., Teixeira, P., & Ferreira, V. (2020). Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains. Food Microbiology, 87, 103351. https://doi.org/10.1016/j.fm.2019.103351pt_PT
dc.identifier.doi10.1016/j.fm.2019.103351pt_PT
dc.identifier.eid85075005743
dc.identifier.issn0740-0020
dc.identifier.pmid31948609
dc.identifier.urihttp://hdl.handle.net/10400.14/28592
dc.identifier.wos000506865300018
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectListeria monocytogenespt_PT
dc.subjectCold-stresspt_PT
dc.subjectSalt-stresspt_PT
dc.subjectInvasionpt_PT
dc.subjectGastro-intestinal tractpt_PT
dc.titleImpact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strainspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood Microbiologypt_PT
oaire.citation.volume87
person.familyNameMagalhães
person.familyNameBrandão
person.familyNamePimentel
person.familyNameTeixeira
person.familyNameBorges Ferreira
person.givenNameRui
person.givenNameTeresa
person.givenNameLígia
person.givenNamePaula
person.givenNameVânia
person.identifierK-1359-2014
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person.identifier.orcid0000-0002-8857-6471
person.identifier.orcid0000-0002-6656-017X
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0001-7093-9193
person.identifier.ridK-1359-2014
person.identifier.ridJ-9418-2012
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person.identifier.ridJ-8678-2014
person.identifier.ridK-1343-2014
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id6602174128
person.identifier.scopus-author-id22035944100
person.identifier.scopus-author-id7005895206
person.identifier.scopus-author-id8317338900
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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