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Microbial degradation of 17β -estradiol and 17α -ethinylestradiol followed by a validated HPLC-DAD method

2010Journal article Restricted access
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dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorCarvalho, Maria F.
dc.contributor.authorAfonso, Carlos M. M.
dc.contributor.authorTiritan, Maria E.
dc.contributor.authorCastro, Paula M.L.
dc.date.accessioned2011-07-29T09:36:13Z
dc.date.available2011-07-29T09:36:13Z
dc.date.issued2010
dc.description.abstractThis work aimed at studying the biodegradation of two estrogens, 17agr -estradiol (E2) and 17β -ethinylestradiol (EE2), and their potential metabolism to estrone (E1) by microbial consortia. The biodegradation studies were followed by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) using a specifically developed and validated method. Biodegradation studies of the estrogens (E2 and EE2) were carried out with activated sludge (consortium A, CA) obtained from a Wastewater Treatment Plant (WWTP) and with a microbial consortium able to degrade recalcitrant compounds, namely fluorobenzene (consortium B, CB). E2 was more extensively degraded than EE2 by CA whereas CB was only able to degrade E2. The addition of acetate as a supplementary carbon source led to a faster biodegradation of E2 and EE2. E1 was detected as a metabolite only during the degradation of E2. The 16S rRNA gene sequence analyses of strains recovered from the degrading cultures revealed the presence of the genera Pseudomonas, Chryseobacterium and Alcaligenes. The genera Pseudomonas and Chryseobacterium were retrieved from cultures supplied with E2 and EE2, while the genus Alcaligenes was found in the presence of E2, suggesting that they might be involved in the degradation of these compounds.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRIBEIRO, Ana R... [et al.] - Microbial Degradation of 17-β-Estradiol and 17-α-Ethinylestradiol Followed by a Validated HPLC-DAD Method. Journal of Environmental Science and Health: Part B - Pesticides, Food Contaminants, and Agricultural Wastes. ISSN 1532-4109. Vol. 45, n.º 4 (2010), p. 265-273por
dc.identifier.doi10.1080/03601231003704523
dc.identifier.eissn1532-4109
dc.identifier.issn0360-1234
dc.identifier.urihttp://hdl.handle.net/10400.14/4849
dc.identifier.wosWOS:000277586700001
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherTaylor & Francispor
dc.relation226/94
dc.relationBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
dc.subjectBiodegradation
dc.subjectEndocrine disrupting chemicals
dc.subject17β-estradiol
dc.subject17α-ethinylestradiol
dc.subjectEstrone
dc.subjectHPLC-DAD
dc.titleMicrobial degradation of 17β -estradiol and 17α -ethinylestradiol followed by a validated HPLC-DAD methodpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOLOGICAL PRODUCTION OF INDUSTRIALLY RELEVANT FLUOROORGANIC COMPOUNDS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44670%2F2008/PT
oaire.citation.endPage273
oaire.citation.issue4
oaire.citation.startPage265
oaire.citation.titleJournal of Environmental Science and Health, Part B
oaire.citation.volume45
person.familyNameRibeiro
person.familyNameCarvalho
person.familyNameAfonso
person.familyNameTiritan
person.familyNameCastro
person.givenNameAna Rita Lado
person.givenNameM. Fátima
person.givenNameCarlos
person.givenNameMaria Elizabeth
person.givenNamePaula
person.identifier903786
person.identifier1310409
person.identifier2013444
person.identifier.ciencia-id421D-DC6E-F6E3
person.identifier.ciencia-id6913-1464-155B
person.identifier.ciencia-id6A10-3565-6158
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-4445-5379
person.identifier.orcid0000-0002-7181-0540
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0003-3320-730X
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridI-5989-2015
person.identifier.ridK-9492-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id57207918943
person.identifier.scopus-author-id14038677200
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id6602890981
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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