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Detection of premature stop codons leading to truncated internalin A among food and clinical strains of Listeria monocytogenes

2017Journal article Restricted access
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dc.contributor.authorSilva, Margarida Ferreira da
dc.contributor.authorFerreira, Vânia
dc.contributor.authorMagalhães, Rui
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorAlves, Artur
dc.contributor.authorTeixeira, Paula
dc.date.accessioned2017-12-17T20:17:05Z
dc.date.available2017-12-17T20:17:05Z
dc.date.issued2017
dc.description.abstractListeria monocytogenes is a food-borne pathogen responsible for outbreaks and sporadic cases of listeriosis, a severe invasive disease. Internalin A (InlA) a protein encoded by inlA has a key role in the mechanism of pathogenesis in L. monocytogenes infection, specifically in the invasion of human intestinal epithelial cells. Studies on inlA have shown that mutations leading to premature stop codons (PMSCs) occur naturally and are associated with impaired virulence of L. monocytogenes strains. Increasing evidence suggests that inlA PMSCs mutations are frequent in strains from foods, but rare among clinical isolates. In this study, 22 L. monocytogenes strains collected in Portugal from the processing environment of a bakery industry (n = 1), different food products (n = 10) and human clinical cases (n = 11) were analysed for mutations in inlA and invasion efficiency in Caco-2 cells. Sequencing revealed previously reported mutations types leading to PMSCs in three food and one clinical strain presenting different molecular serotypes (i.e., IIa, IIb and IIc). The remaining 18 isolates did not show PMSCs in inlA. The four strains with PMSCs in inlA presented lower invasiveness efficiencies in Caco-2 cells (below 8.9%) when compared to the control strain (full-length InlA). In addition, one clinical isolate showed reduced invasion efficiency but no PMSCs in inlA. This isolate showed increased inlA transcript levels to that obtained for the laboratory control strain. Our data support the hypothesis that L. monocytogenes isolated from food have attenuated invasion due to the presence of inlA PMSCs. This information would be critically needed for adequate risk-assessments of the foodborne illness burden associated with L. monocytogenes strains.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, M. F., Ferreira, V., Magalhaes, R., Almeida, G., Alves, A., Teixeira, P. (2017). Detection of premature stop codons leading to truncated internalin A among food and clinical strains of listeria monocytogenes. Food Microbiology, 63, 6-11pt_PT
dc.identifier.doi10.1016/j.fm.2016.10.033pt_PT
dc.identifier.eid84994061023
dc.identifier.eissn1095-9998
dc.identifier.issn0740-0020
dc.identifier.pmid28040183
dc.identifier.urihttp://hdl.handle.net/10400.14/23689
dc.identifier.wos000392355500002
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-07-0124-FEDER-000052pt_PT
dc.relationCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
dc.subjectListeria monorytogenespt_PT
dc.subjectInlApt_PT
dc.subjectInternalin Apt_PT
dc.subjectPremature stop codons (PMSCs)pt_PT
dc.subjectCaco-2pt_PT
dc.subjectInvasion assaypt_PT
dc.titleDetection of premature stop codons leading to truncated internalin A among food and clinical strains of Listeria monocytogenespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERISTICS OF SPECIFIC STRAINS OF LISTERIA MONOCYTOGENES POTENTIATING PERSISTENCE IN FOOD AND FOOD PROCESSING ENVIRONMENTS AND RELEVANCE TO FOOD SAFETY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71704%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72617%2F2010/PT
oaire.citation.endPage11pt_PT
oaire.citation.startPage6pt_PT
oaire.citation.titleFood Microbiologypt_PT
oaire.citation.volume63pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameBorges Ferreira
person.familyNameMagalhães
person.familyNameAlmeida
person.familyNameAlves
person.familyNameTeixeira
person.givenNameMargarida
person.givenNameVânia
person.givenNameRui
person.givenNameGonçalo
person.givenNameArtur
person.givenNamePaula
person.identifierK-1359-2014
person.identifier267767
person.identifier84187
person.identifier.ciencia-id4C1A-BDD4-D716
person.identifier.ciencia-id341E-5F76-548C
person.identifier.ciencia-id2110-7954-9D7A
person.identifier.orcid0000-0001-8858-2516
person.identifier.orcid0000-0001-7093-9193
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0003-1513-0951
person.identifier.orcid0000-0003-0117-2958
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridK-1343-2014
person.identifier.ridK-1359-2014
person.identifier.ridK-4389-2014
person.identifier.ridC-3645-2008
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id8317338900
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id7003759404
person.identifier.scopus-author-id7103319531
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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